Bcl-2 overexpression prevents calcium overload and subsequent apoptosis in dystrophic myotubes

被引:48
作者
Basset, O
Boittin, FX
Cognard, C
Constantin, B
Ruegg, UT
机构
[1] Univ Geneva, Geneva Lausanne Sch Pharmaceut Sci, Pharmacol Lab, CH-1211 Geneva 4, Switzerland
[2] Univ Poitiers, CNRS, UMR 6187, Inst Physiol & Biol Cellulaire, F-86022 Poitiers, France
关键词
aequorin; apoptosis; Bcl-2; Duchenne muscular dystrophy; inositol 1,4,5-trisphosphite receptor; staurosporine;
D O I
10.1042/BJ20051265
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Duchenne muscular dystrophy (DMD) is a lethal disease caused by the lack of the cytoskeletal protein dystrophin. Altered calcium homoeostasis and increased calcium concentrations in dystrophic fibres may be responsible for the degeneration of muscle occurring in DMD. In the present study, we used subsarcolemmal and mitochondrial-targeted aequorin to study the effect of the antiapoptotic Bcl-2 protein overexpression on carbachol-induced near-plasma membrane and mitochondrial calcium responses in myotubes derived from control C57 and dystrophic (mdx) mice. We show that Bcl-2 overexpression decreases subsarcolemmal and mitochondrial calcium overload that occurs during activation of nicotinic acetylcholine receptors in dystrophic myotubes. Moreover, our results suggest that overexpressed Bcl-2 protein may prevent near-plasma membrane and mitochondrial calcium overload by inhibiting IP(3)Rs (inositol 1,4,5-trisphosphate receptors), which we have shown previously to be involved in abnormal calcium homoeostasis in dystrophic myotubes. Most likely as a consequence, the inhibition of IP3R function by Bcl-2 also inhibits calcium-dependent apoptosis in these cells.
引用
收藏
页码:267 / 276
页数:10
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