Developmental regulation of leptin gene expression in rat brain and pituitary

被引:38
作者
Morash, B
Wilkinson, D
Murphy, P
Ur, E
Wilkinson, M [1 ]
机构
[1] Dalhousie Univ, IWK Grace Hlth Ctr, Dept Obstet, Halifax, NS B3H 2Y9, Canada
[2] Dalhousie Univ, IWK Grace Hlth Ctr, Div Endocrinol, Halifax, NS B3H 2Y9, Canada
[3] Dalhousie Univ, IWK Grace Hlth Ctr, Dept Gynaecol, Halifax, NS B3H 2Y9, Canada
[4] Dalhousie Univ, IWK Grace Hlth Ctr, Dept Physiol, Halifax, NS B3H 2Y9, Canada
[5] Dalhousie Univ, IWK Grace Hlth Ctr, Dept Biophys, Halifax, NS B3H 2Y9, Canada
关键词
ontogeny; leptin receptors; RT-PCR; immunohistochemistry;
D O I
10.1016/S0303-7207(01)00626-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have previously reported the expression of leptin mRNA and protein in adult rat brain and pituitary gland. We report here the presence of leptin and leptin receptor mRNA in neonatal female rat brain and pituitary using RT-PCR as well as leptin and leptin receptor immuno reactivity in neonatal rat brain. In addition, we describe age-related changes in leptin mRNA expression in female rat brain and pituitary from postnatal day 2 to 28, evaluated using semi-quantitative RT-PCR analysis. Age-related differences in leptin (ob) mRNA levels were tissue-dependent. The most striking developmental changes were noted in the pituitary and cerebral cortex. In the pituitary, ob mRNA levels were maximal during postnatal days 7-14 and fell sharply by postnatal day 22. In cortex, ob mRNA levels were low in neonatal pups (day 2-7) but increased significantly between postnatal days 14 and 28. Leptin mRNA was detectable at postnatal day 2 in hypothalamus and subcutaneous fat. No significant differences in the level of expression were observed between postnatal day 2 and 28. Serum leptin levels were highest at day 7-14 and decreased significantly by day 21-28, coincident with the fall in pituitary leptin expression. The high levels of leptin expression in the neonatal pituitary suggest that this gland may contribute to the circulating leptin levels during early postnatal development, when adipose deposits are minimal. These data indicate that regulation of leptin gene expression in the postnatal period is tissue-dependent, a finding, which suggests that local leptin expression may have important functional significance in the development of the brain-pituitary system. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:151 / 159
页数:9
相关论文
共 49 条
[1]   Leptin regulation of neuroendocrine systems [J].
Ahima, RS ;
Saper, CB ;
Flier, JS ;
Elmquist, JK .
FRONTIERS IN NEUROENDOCRINOLOGY, 2000, 21 (03) :263-307
[2]   Regulation of neuronal and glial proteins by leptin:: Implications for brain development [J].
Ahima, RS ;
Bjorbæk, C ;
Osei, S ;
Flier, JS .
ENDOCRINOLOGY, 1999, 140 (06) :2755-2762
[3]   Postnatal leptin surge and regulation of circadian rhythm of leptin by feeding - Implications for energy homeostasis and neuroendocrine function [J].
Ahima, RS ;
Prabakaran, D ;
Flier, JS .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (05) :1020-1027
[4]   The stomach is a source of leptin [J].
Bado, A ;
Levasseur, S ;
Attoub, S ;
Kermorgant, S ;
Laigneau, JP ;
Bortoluzzi, MN ;
Moizo, L ;
Lehy, T ;
Guerre-Millo, M ;
Le Marchand-Brustel, Y ;
Lewin, MJM .
NATURE, 1998, 394 (6695) :790-793
[5]   A role for leptin and its cognate receptor in hematopoiesis [J].
Bennett, BD ;
Solar, GP ;
Yuan, JQ ;
Mathias, J ;
Thomas, GR ;
Matthews, W .
CURRENT BIOLOGY, 1996, 6 (09) :1170-1180
[6]   ALTERED NEUROANATOMICAL ORGANIZATION IN THE CENTRAL NERVOUS-SYSTEM OF THE GENETICALLY-OBESE (OB-OB) MOUSE [J].
BEREITER, DA ;
JEANRENAUD, B .
BRAIN RESEARCH, 1979, 165 (02) :249-260
[7]   Divergent signaling capacities of the long and short isoforms of the leptin receptor [J].
Bjorbaek, C ;
Uotani, S ;
da Silva, B ;
Flier, JS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (51) :32686-32695
[8]   The long form of the leptin receptor (OB-Rb) is widely expressed in the human brain [J].
Burguera, B ;
Couce, ME ;
Long, J ;
Lamsam, J ;
Laakso, K ;
Jensen, MD ;
Parisi, JE ;
Lloyd, RV .
NEUROENDOCRINOLOGY, 2000, 71 (03) :187-195
[9]   Neuroendocrine regulation and actions of leptin [J].
Casanueva, FF ;
Dieguez, C .
FRONTIERS IN NEUROENDOCRINOLOGY, 1999, 20 (04) :317-363
[10]   Expression of OB receptor splice variants during prenatal development of the mouse [J].
Chen, SC ;
Cunningham, JJ ;
Smeyne, RJ .
JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH, 2000, 20 (01) :87-103