Characterization of Cryptosporidium parvum in human and animal feces by single-tube nested polymerase chain reaction and restriction analysis

被引：38

作者：

Homan, W ^{[1
]}

van Gorkom, T ^{[1
]}

Kan, YY ^{[1
]}

Hepener, J ^{[1
]}

机构：

[1] Natl Inst Publ Hlth & Environm, Microbiol Lab Hlth Protect, NL-3720 BA Bilthoven, Netherlands

来源：

PARASITOLOGY RESEARCH | 1999年 / 85卷 / 8-9期

关键词：

D O I：

10.1007/s004360050619

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A DNA isolation and purification method is described that produced DNA free of inhibitory substances in 148 of the 159 analyzed fecal samples. The polymerase chain reaction (PCR) product from a sensitive single-tube nested PCR that amplifies a part of an oocyst protein was used to characterize Cryptosporidium parvum genotypes by a simple restriction analysis. Genotype 1 was solely detected in human-derived oocysts, genotype 2 was present in both animal and human-derived oocysts. The ratio between both genotypes in humans in The Netherlands varied markedly between samples obtained during a period of augmented cases of cryptosporidiosis in the western part of the country and randomly selected samples from gastroenteritis patients. Sequence analysis of a 581-bp fragment from the nested PCR product revealed 12 nucleotide substitutions between the two genotypes. Sequences from isolates in each genotype group were identical.

引用

页码：707 / 712

页数：6

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