The cytotoxicity of cadmium based, aqueous phase - Synthesized, quantum dots and its modulation by surface coating

被引：286

作者：

Su, Yuanyuan ^{[1
,2
]}

He, Yao ^{[1
,3
]}

Lu, Haoting ^{[3
]}

Sai, Liman ^{[3
]}

Li, Qingnuan ^{[1
]}

Li, Wenxin ^{[1
]}

Wang, Lianhui ^{[3
]}

Shen, Pingping ^{[2
]}

Huang, Qing ^{[1
]}

Fan, Chunhai ^{[1
,4
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

[3] Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China

[4] Shanghai Jiao Tong Univ, Bio X Life Sci Res Ctr, Shanghai 200030, Peoples R China

来源：

BIOMATERIALS | 2009年 / 30卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Aqueous phase; Biocompatible materials; Cytotoxicity; Core-shell-shell quantum dots; CDTE NANOCRYSTALS; CDSE; PHOTOLUMINESCENCE; TOXICITY; GROWTH;

D O I：

10.1016/j.biomaterials.2008.09.029

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this report, we evaluated the cytotoxicity of a series of quantum dots (QDs) directly synthesized in aqueous phase, i.e., thiols-stabilized CdTe, CdTe/CdS core-shell structured and CdTe/CdS/ZnS core-shell-shell structured QDs, with a variety of cell lines including K562 and HEK293T. We have demonstrated that the CdTe QDs are highly toxic for cells due to the release of cadmium ions. Epitaxial growth of a CdS layer reduces the cytotoxicity of QDs to a small extent. However, the presence of a ZnS outlayer greatly improves the biocompatibility of QDs, with no observed cytotoxicity even at very high concentration and long-time exposure in cells. Our systematic investigation clearly shows that the cytotoxicity of QDs can be modulated through elaborate surface coatings and that the CdTe/CdS/ZnS core-shell-shell structured QDs directly synthesized in aqueous phase are highly promising biological fluorescent probes for cellular imaging. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：19 / 25

页数：7

共 43 条

[1] The use of nanocrystals in biological detection [J].

Alivisatos, P .

NATURE BIOTECHNOLOGY, 2004, 22 (01) :47-52

[2] Alloyed semiconductor quantum dots: Tuning the optical properties without changing the particle size [J].

Bailey, RE ;

Nie, SM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (23) :7100-7106

[3] Enhancement effect of illumination on the photoluminescence of water-soluble CdTe nanocrystals: Toward highly fluorescent CdTe/CdS core-shell structure [J].

Bao, HB ;

Gong, YJ ;

Li, Z ;

Gao, MY .

CHEMISTRY OF MATERIALS, 2004, 16 (20) :3853-3859

[4] Comparison of electrokinetic properties of colloidal fullerenes (n-C60) formed using two procedures [J].

Brant, J ;

Lecoanet, H ;

Hotze, M ;

Wiesner, M .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2005, 39 (17) :6343-6351

[5] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[6] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[7] (CdSe)ZnS core-shell quantum dots: Synthesis and characterization of a size series of highly luminescent nanocrystallites [J].

Dabbousi, BO ;

RodriguezViejo, J ;

Mikulec, FV ;

Heine, JR ;

Mattoussi, H ;

Ober, R ;

Jensen, KF ;

Bawendi, MG .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (46) :9463-9475

[8]

Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334

[9] In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].

Dubertret, B ;

Skourides, P ;

Norris, DJ ;

Noireaux, V ;

Brivanlou, AH ;

Libchaber, A .

SCIENCE, 2002, 298 (5599) :1759-1762

[10] Strongly photoluminescent CdTe nanocrystals by proper surface modification [J].

Gao, MY ;

Kirstein, S ;

Möhwald, H ;

Rogach, AL ;

Kornowski, A ;

Eychmüller, A ;

Weller, H .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (43) :8360-8363

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