Combining two-photon excitation with fluorescence lifetime imaging

被引:10
作者
Gerritsen, HC [1 ]
Vroom, JM [1 ]
de Grauw, CJ [1 ]
机构
[1] Univ Utrecht, Debye Inst, Utrecht, Netherlands
来源
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE | 1999年 / 18卷 / 05期
关键词
D O I
10.1109/51.790989
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The in-depth imaging properties of two-photon excitation (TPE) imaging are discussed. In particular, a comparison is made between the in-depth imaging properties of confocal laser scanning microscopy (CLSM) and TPE microscopy, using dental biofilm as the test specimen. Two measurements are carried out, either at one excitation and two emission wavelengths or at two excitation and one emission wavelength. Results from these measurements reveal that TPE imaging with a microscope objective that is properly matched to the sample results in a two- to four-times larger penetration depth than with CLSM.
引用
收藏
页码:31 / 36
页数:6
相关论文
共 25 条
[1]   AN ERROR ANALYSIS OF THE RAPID LIFETIME DETERMINATION METHOD FOR THE EVALUATION OF SINGLE EXPONENTIAL DECAYS [J].
BALLEW, RM ;
DEMAS, JN .
ANALYTICAL CHEMISTRY, 1989, 61 (01) :30-33
[2]   A modified chemostat system to study the ecology of oral biofilms [J].
Bradshaw, DJ ;
Marsh, PD ;
Schilling, KM ;
Cummins, D .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 80 (02) :124-130
[3]   FLUORESCENCE LIFETIME IMAGING USING A CONFOCAL LASER SCANNING MICROSCOPE [J].
BUURMAN, EP ;
SANDERS, R ;
DRAAIJER, A ;
GERRITSEN, HC ;
VANVEEN, JJF ;
HOUPT, PM ;
LEVINE, YK .
SCANNING, 1992, 14 (03) :155-159
[4]   ANATOMICAL AND FUNCTIONAL IMAGING OF NEURONS USING 2-PHOTON LASER-SCANNING MICROSCOPY [J].
DENK, W ;
DELANEY, KR ;
GELPERIN, A ;
KLEINFELD, D ;
STROWBRIDGE, BW ;
TANK, DW ;
YUSTE, R .
JOURNAL OF NEUROSCIENCE METHODS, 1994, 54 (02) :151-162
[5]   Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing [J].
French, T ;
So, PTC ;
Weaver, DJ ;
CoelhoSampaio, T ;
Gratton, E ;
Voss, EW ;
Carrero, J .
JOURNAL OF MICROSCOPY-OXFORD, 1997, 185 :339-353
[6]   REFRACTIVE-INDEX-INDUCED ABERRATIONS IN 2-PHOTON CONFOCAL FLUORESCENCE MICROSCOPY [J].
JACOBSEN, H ;
HANNINEN, P ;
SOINI, E ;
HELL, SW .
JOURNAL OF MICROSCOPY-OXFORD, 1994, 176 :226-230
[7]  
KUSELER A, 1993, CARIES RES, V27, P183
[8]   FLUORESCENCE LIFETIME IMAGING OF CALCIUM USING QUIN-2 [J].
LAKOWICZ, JR ;
SZMACINSKI, H ;
NOWACZYK, K ;
JOHNSON, ML .
CELL CALCIUM, 1992, 13 (03) :131-147
[9]   LIFETIME-SELECTIVE FLUORESCENCE IMAGING USING AN RF PHASE-SENSITIVE CAMERA [J].
LAKOWICZ, JR ;
BERNDT, KW .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1991, 62 (07) :1727-1734
[10]  
LAKOWICZ JR, 1992, SPIE, V1640, P390