Expression of homeobox-containing genes in human preimplantation development and in embryos with chromosomal aneuploidies

被引:16
作者
Kuliev, A
Kukharenko, V
Morozov, G
Freidine, M
Rechitsky, S
Verlinsky, O
Ivakhnenko, V
Gindilis, V
Strom, C
Verlinsky, Y
机构
[1] Reproductive Genetics Institute, Chicago, IL
[2] National Research Center for Medical Genetics, RAMS, Moscow
关键词
homeobox; preimplantation development; aneuploidy; triploidy; spontaneously aborted embryos;
D O I
10.1007/BF02072541
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Purpose: The purpose of the study was to investigate homeobox gene expression in human oocytes and preembryos and in postimplantation embryos with impaired embryonic development determined by chromosomal abnormalities. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) with intron spanning primer sets for Homeobox gene sequences was used. Results: The homeobox genes HoxA4, HoxA7, HoxB4, and HoxB5 were present in human oocytes and cleaving normal and triploid embryos. The expression pattern was different between chromosomally abnormal and normal first-trimester embryos. Of four homeobox transcripts (HoxA7, HoxB4, HoxB5, and HoxC6) that are expressed in diploid embryos, only HoxA7, HoxB4, and HoxC6 were present in a trisomy 7 embryo, and only HoxB4 and HoxB5 in triploid embryos and an embryo with trisomy 9. Cloning experiments revealed differences in the number of homeobox clones obtained from trisomy 7 and control embryos. Conclusions: The transcipts of homeobox genes, HoxA4 HoxA7, HoxB4, and HoxB5, were present in oocytes and cleaving embryos. The pattern of expression of homeobox genes in cultured fibroblasts derived from spontaneously aborted embryos with aneuploidies was different from that in control diploid cells.
引用
收藏
页码:177 / 181
页数:5
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