A model system to provide a good in vitro simulation of biological mineralization.

被引：32

作者：

Dorozhkin, SV ^{[1
]}

Dorozhkina, EI ^{[1
]}

Epple, M ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Fac Chem, D-44780 Bochum, Germany

来源：

CRYSTAL GROWTH & DESIGN | 2004年 / 4卷 / 02期

关键词：

D O I：

10.1021/cg034066s

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

The in vivo process of biological mineralization can be successfully simulated in vitro by a slow crystallization of a nonstoichiometric carbonated apatite from revised simulated body fluid (rSBF) carried out in a constant-composition double-diffusion (CCDD) device under physiological conditions (temperature 37 degreesC, solution pH within 7.2-7.4). The substitution of an organic buffer (Hepes) by permanent carbon dioxide bubbling to keep the pH constant has no strong influence on the crystallization. By increasing the overall concentration, calcification can be easily induced on porous bioinert surfaces such as cellulose or cholesterol that normally cannot be coated with calcium phosphates from supersaturated solutions without additional promoters.

引用

页码：389 / 395

页数：7

共 44 条

[1]

HYDROXYAPATITE - MECHANISM OF FORMATION AND PROPERTIES [J].