Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy

被引：72

作者：

Kannan, Balakrishnan

Har, Jia Yi

Liu, Ping

Maruyama, Ichiro

Ding, Jeak Ling

Wohland, Thorsten

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[3] Singapore MIT Alliance, Singapore 117576, Singapore

[4] Genome Inst Singapore, Singapore 138672, Singapore

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 10期

关键词：

D O I：

10.1021/ac0600959

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fluorescence correlation spectroscopy (FCS) setup is built with an electron multiplying charge-coupled device camera. Although the instrument has a limited time resolution of 4 ms, compared to 0.1-0.2 mu s for common instruments using avalanche photodiodes, it allows multiplexing of FCS measurements, has a software-adjustable pinhole after data collection, performs flow speed as well as flow direction measurements in microchannels and could be used to do spectral FCS. Measurements are performed on fluorescent dyes and polystyrene beads in high-viscosity media and on epidermal growth factor receptors in Chinese hamster ovary cells. Using real measurements on single spots, multiplexing of focal spots and detection elements are simulated and the results are discussed.

引用

页码：3444 / 3451

页数：8

共 23 条

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