Selective adherence of non-typeable Haemophilus influenzae (NTHi) to mucus of epithelial cells in the chinchilla Eustachian tube and middle ear

被引:44
作者
Miyamoto, N [1 ]
Bakaletz, LO [1 ]
机构
[1] OHIO STATE UNIV,OTOL RES LABS,DEPT OTOLARYNGOL,COLL MED,COLUMBUS,OH 43210
关键词
fimbriae; otitis media; chinchilla; Eustachian tube; middle ear epithelium; mucolytic agent;
D O I
10.1006/mpat.1996.0067
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Frozen sections of chinchilla Eustachian tube (ET) and middle ear mucosa were incubated with either FITC-labeled non-typeable Haemophilus influenzae (NTHi) or Bordetella pertussis. The number of bacteria adherent to ''roof'' vs ''floor'' regions was compared for each of three anatomic portions of the ET and for middle ear epithelium noting whether bacteria adhered to mucus or to epithelial cells. NTHi strains adhered significantly greater to mucus in the ET lumen whereas B. pertussis preferentially adhered to epithelial cells lining the ET (P less than or equal to 0.05). A non-fimbriated isogenic mutant of NTHi adhered significantly less to mucus than the parental isolate at all sites of the ET floor (P less than or equal to 0.05). Isolated fimbrin protein adhered to ET mucus and blocked adherence of whole organisms. Treatment with the mucolytic agent N-acetyl-L-cysteine resulted in significantly reduced adherence of NTHi to mucus (P less than or equal to 0.001) and eliminated the ability to detect binding of isolated fimbrin protein. N-acetyl-L-cysteine treatment did not affect adherence of either B. pertussis or NTHi to epithelial cells. These data indicated that NTHi may mediate ascension of the ET from the nasopharynx primarily via adherence to and growth in mucus overlying the floor region of the tubal lumen. The OMP P5-homologous fimbriae were shown to contribute to this binding. (C) 1996 Academic Press Limited
引用
收藏
页码:343 / 356
页数:14
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