Pseudomonas putida KT2440 responds specifically to chlorophenoxy herbicides and their initial metabolites

被引:48
作者
Benndorf, Dirk [1 ]
Thiersch, Markus [1 ]
Loffhagen, Norbert [1 ]
Kunath, Christfried [1 ]
Harms, Hauke [1 ]
机构
[1] UFZ Helmholtz Ctr Environm Res, Ctr Environm Res, Dept Environm Microbiol, D-04301 Leipzig, Germany
关键词
chemostress; 2-D database; multiple drug resistance; oxidative stress; resistance mechanism;
D O I
10.1002/pmic.200500781
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Pseudomonas putida KT2440 is often used as a model to investigate toxicity mechanisms and adaptation to hazardous chemicals in bacteria. The objective of this paper was to test the impact of the chlorophenoxy herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-(2,4-dichlorophenoxy)propanoic acid (DCPP) and their metabolites 2,4-dichlorophenol (DCP) and 3,5-dichlorocatechol. (DCC), on protein expression patterns and physiological parameters. Both approaches showed that DCC has a different mode of action and induces different responses than DCPP, 2,4-D and DCP. DCC was the most toxic compound and was active as an uncoupler of oxidative phosphorylation. It repressed the synthesis of ferric uptake regulator (Fur)-dependent proteins, e.g. fumarase C and L-ornithine N5-oxygenase, which are involved in oxidative stress response and iron uptake. DCPP, 2,4-D and DCP were less toxic than DCC. They disturbed oxidative phosphorylation to a lesser extent by a yet unknown mechanism. Furthermore, they repressed enzymes of energy-consuming biosynthetic pathways and induced membrane transporters for organic substrates. A TolC homologue component of multidrug resistance transporters was found to be induced, which is probably involved in the removal of lipophilic compounds from membranes.
引用
收藏
页码:3319 / 3329
页数:11
相关论文
共 48 条
[1]   Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12 [J].
Aono, R ;
Tsukagoshi, N ;
Yamamoto, M .
JOURNAL OF BACTERIOLOGY, 1998, 180 (04) :938-944
[2]   Regulation of catabolic enzymes during long-term exposure of Delftia acidovorans MC1 to chlorophenoxy herbicides [J].
Benndorf, D ;
Davidson, I ;
Babel, W .
MICROBIOLOGY-SGM, 2004, 150 :1005-1014
[3]   Assimilatory detoxification of herbicides by Delftia acidovorans MC1:: induction of two chlorocatechol 1,2-dioxygenases as a response to chemostress [J].
Benndorf, D ;
Babel, W .
MICROBIOLOGY-SGM, 2002, 148 :2883-2888
[4]  
Benndorf D, 1999, ELECTROPHORESIS, V20, P781, DOI 10.1002/(SICI)1522-2683(19990101)20:4/5<781::AID-ELPS781>3.0.CO
[5]  
2-A
[6]   Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress [J].
Benndorf, D ;
Loffhagen, N ;
Babel, W .
FEMS MICROBIOLOGY LETTERS, 2001, 200 (02) :247-252
[7]   FROM XENOBIOTIC TO ANTIBIOTIC, FORMATION OF PROTOANEMONIN FROM 4-CHLOROCATECHOL BY ENZYMES OF THE 3-OXOADIPATE PATHWAY [J].
BLASCO, R ;
WITTICH, RM ;
MALLAVARAPU, M ;
TIMMIS, KN ;
PIEPER, DH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) :29229-29235
[8]   IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS [J].
BLUM, H ;
BEIER, H ;
GROSS, HJ .
ELECTROPHORESIS, 1987, 8 (02) :93-99
[9]   Toxicity of mono-, di- and tri-chlorophenols to lux marked terrestrial bacteria, Burkholderia species Rasc c2 and Pseudomonas fluorescens [J].
Boyd, EM ;
Killham, K ;
Meharg, AA .
CHEMOSPHERE, 2001, 43 (02) :157-166
[10]  
CROSS J. E., 1966, PLANT DB REPORT, V50, P557