Two potent competitive inhibitors discriminating α-glucosidase family I from family II

被引：83

作者：

Kimura, A ^{[1
]}

Lee, JH

Lee, IS

Lee, HS

Park, KH

Chiba, S

Kim, D

机构：

[1] Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan

[2] Chonnam Natl Univ, Engn Res Inst, Gwangju 500757, South Korea

[3] Chonnam Natl Univ, Fac Appl Chem Engn, Gwangju 500757, South Korea

[4] Seoul Natl Univ, Ctr Agr Biomat, Natl Lab Funct Food Carbohydrates, Suwon 441744, South Korea

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

enzyme inhibitor; alpha-glucosidase family; isoacarbose; acarviosine-glucose; acarbose;

D O I：

10.1016/j.carres.2003.10.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inhibition kinetics for isoacarbose (a pseudotetrasaccharide, IsoAca) and acarviosine-glucose (pseudotrisaccharide, AcvGlc), both of which are derivatives of acarbose, were investigated with various types of alpha-glucosidases obtained from microorganisms, plants, and insects. IsoAca and AcvGlc, competitive inhibitors, allowed classification of alpha-glucosidases into two groups. Enzymes of the first group were strongly inhibited by AcvGlc and weakly by lsoAca, in which the K-i values of AcvGlc (0.35-3.0 PM) were 21 - to 440-fold smaller than those of lsoAca. However, the second group of enzymes showed similar Ki values, ranging from 1.6 to 8.0 muM for both compounds. This classification for alpha-glucosidases is in total agreement with that based on the similarity of their amino acid sequences (family I and family II). This indicated that the alpha-glucosidase families I and II could be clearly distinguished based on their inhibition kinetic data for lsoAca and AcvGlc. The two groups of alpha-glucosidases seemed to recognize distinctively the extra reducing-terminal glucose unit in IsoAca. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1035 / 1040

页数：6

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