Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs

被引：73

作者：

Yutin, N

Suzuki, MT

Béjà, O ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel

[2] Univ Maryland, Ctr Environm Sci, Chesapeake Biol Lab, Solomons, MD 20688 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 12期

关键词：

D O I：

10.1128/AEM.71.12.8958-8962.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.

引用

收藏

页码：8958 / 8962

页数：5

相关论文

共 18 条

[1] Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments [J].

Achenbach, LA ;

Carey, J ;

Madigan, MT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :2922-2926

[2] Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats [J].

Allgaier, M ;

Uphoff, H ;

Felske, A ;

Wagner-Döbler, I .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (09) :5051-5059

[3] Unsuspected diversity among marine aerobic anoxygenic phototrophs [J].

Béjà, O ;

Suzuki, MT ;

Heidelberg, JF ;

Nelson, WC ;

Preston, CM ;

Hamada, T ;

Eisen, JA ;

Fraser, CM ;

DeLong, EF .

NATURE, 2002, 415 (6872) :630-633

[4] Bacteriochlorophyll a in the ocean:: Is anoxygenic bacterial photosynthesis important? [J].

Goericke, R .

LIMNOLOGY AND OCEANOGRAPHY, 2002, 47 (01) :290-295

[5] Remarkable diversity of phototrophic purple bacteria in a permanently frozen Antarctic lake [J].

Karr, EA ;

Sattley, WM ;

Jung, DO ;

Madigan, MT ;

Achenbach, LA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) :4910-4914

[6] Bacterial photosynthesis in surface waters of the open ocean [J].

Kolber, ZS ;

Van Dover, CL ;

Niederman, RA ;

Falkowski, PG .

NATURE, 2000, 407 (6801) :177-179

[7] Contribution of aerobic photoheterotrophic bacteria to the carbon cycle in the ocean [J].

Kolber, ZS ;

Plumley, FG ;

Lang, AS ;

Beatty, JT ;

Blankenship, RE ;

VanDover, CL ;

Vetriani, C ;

Koblizek, M ;

Rathgeber, C ;

Falkowski, PG .

SCIENCE, 2001, 292 (5526) :2492-2495

[8] ARB:: a software environment for sequence data [J].

Ludwig, W ;

Strunk, O ;

Westram, R ;

Richter, L ;

Meier, H ;

Yadhukumar ;

Buchner, A ;

Lai, T ;

Steppi, S ;

Jobb, G ;

Förster, W ;

Brettske, I ;

Gerber, S ;

Ginhart, AW ;

Gross, O ;

Grumann, S ;

Hermann, S ;

Jost, R ;

König, A ;

Liss, T ;

Lüssmann, R ;

May, M ;

Nonhoff, B ;

Reichel, B ;

Strehlow, R ;

Stamatakis, A ;

Stuckmann, N ;

Vilbig, A ;

Lenke, M ;

Ludwig, T ;

Bode, A ;

Schleifer, KH .

NUCLEIC ACIDS RESEARCH, 2004, 32 (04) :1363-1371

[9] Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel [J].

Massana, R ;

Murray, AE ;

Preston, CM ;

DeLong, EF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :50-56

[10] Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria [J].

Nagashima, KVP ;

Hiraishi, A ;

Shimada, K ;

Matsuura, K .

JOURNAL OF MOLECULAR EVOLUTION, 1997, 45 (02) :131-136