Cortical bone growth and maturational changes in dwarf rats induced by recombinant human growth hormone

被引:15
作者
Martinez, DA
Orth, MW
Carr, KE
Vanderby, R
Vailas, AC
机构
[1] UNIV WISCONSIN, BIODYNAM LAB, MADISON, WI 53706 USA
[2] UNIV WISCONSIN, DEPT ORTHOPAED SURG, MADISON, WI 53792 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1996年 / 270卷 / 01期
关键词
collagen cross-links; hydroxyapatite; density; porosity; collagen accretion; extracellular matrix;
D O I
10.1152/ajpendo.1996.270.1.E51
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The growth hormone (GH)-deficient dwarf rat was used to investigate recombinant human (rh) GH-induced bone formation and to determine whether rhGH facilitates simultaneous increases in bone formation and bone maturation during rapid growth. Twenty dwarf rats, 37 days of age, were randomly assigned to dwarf plus rhGH (GH; n = 10) and dwarf plus vehicle (n = 10) groups. The GH group received 1.25 mg rhGH/kg body wt two times daily for 14 days. Biochemical, morphological, and X-ray diffraction measurements were performed on the femur middiaphysis. rhGH stimulated new bone growth in the GH group, as demonstrated by significant increases (P < 0.05) in longitudinal bone length (6%), middiaphyseal cross-sectional area (20%), and the amount of newly accreted bone collagen (28%) in the total pool of middiaphyseal bone collagen. Cortical bone density, mean hydroxyapatite crystal size, and the calcium and collagen contents (mu g/mm(3)) were significantly smaller in the GH group (P < 0.05). Our findings suggest that the processes regulating new collagen accretion, bone collagen maturation, and mean hydroxyapatite crystal size may be independently regulated during rapid growth.
引用
收藏
页码:E51 / E59
页数:9
相关论文
共 42 条
[1]  
ASPENBERG P, 1985, ACTA ANAT, V121, P84
[2]   OSTEOBLAST-SPECIFIC EXPRESSION OF GROWTH-HORMONE STIMULATES BONE-GROWTH IN TRANSGENIC MICE [J].
BAKER, AR ;
HOLLINGSHEAD, PG ;
PITTSMEEK, S ;
HANSEN, S ;
TAYLOR, R ;
STEWART, TA .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (12) :5541-5547
[3]   THE ONTOGENY OF GROWTH-HORMONE RECEPTORS IN THE RABBIT TIBIA [J].
BARNARD, R ;
HAYNES, KM ;
WERTHER, GA ;
WATERS, MJ .
ENDOCRINOLOGY, 1988, 122 (06) :2562-2569
[4]   CHEMICAL ANATOMY OF BONE .I. A COMPARATIVE STUDY OF BONE COMPOSITION IN 16 VERTEBRATES [J].
BILTZ, RM ;
PELLEGRINO, ED .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1969, A 51 (03) :456-+
[5]   NORMAL MATURATIONAL CHANGES IN BONE-MATRIX, MINERAL, AND CRYSTAL SIZE IN THE RAT [J].
BURNELL, JM ;
TEUBNER, EJ ;
MILLER, AG .
CALCIFIED TISSUE INTERNATIONAL, 1980, 31 (01) :13-19
[6]   GROWTH HORMONE-DEFICIENT DWARFISM IN THE RAT - A NEW MUTATION [J].
CHARLTON, HM ;
CLARK, RG ;
ROBINSON, ICAF ;
GOFF, AEP ;
COX, BS ;
BUGNON, C ;
BLOCH, BA .
JOURNAL OF ENDOCRINOLOGY, 1988, 119 (01) :51-&
[7]   A CONTRIBUTION WITH REVIEW TO THE DESCRIPTION OF MINERALIZATION OF BONE AND OTHER CALCIFIED TISSUES INVIVO [J].
CHRISTOFFERSEN, J ;
LANDIS, WJ .
ANATOMICAL RECORD, 1991, 230 (04) :435-450
[8]   INTRAVENOUS GROWTH-HORMONE - GROWTH-RESPONSES TO PATTERNED INFUSIONS IN HYPOPHYSECTOMIZED RATS [J].
CLARK, RG ;
JANSSON, JO ;
ISAKSSON, O ;
ROBINSON, ICAF .
JOURNAL OF ENDOCRINOLOGY, 1985, 104 (01) :53-61
[9]  
CLARK RG, 1988, J PHYSIOL-LONDON, V398, pP80
[10]   IMMATURE TENDON ADAPTATION TO STRENUOUS EXERCISE [J].
CURWIN, SL ;
VAILAS, AC ;
WOOD, J .
JOURNAL OF APPLIED PHYSIOLOGY, 1988, 65 (05) :2297-2301