Phage HK022 Roi protein inhibits phage lytic growth in Escherichia coli integration host factor mutants

被引:13
作者
Clerget, M
Boccard, F
机构
[1] INST BIOL STRUCT & MICROBIOL,CNRS,CHIM BACTERIENNE LAB,UPR 9043,F-13009 MARSEILLE,FRANCE
[2] UNIV GENEVA,DEPT BIOL MOLEC,CH-1211 GENEVA 4,SWITZERLAND
关键词
D O I
10.1128/jb.178.14.4077-4083.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Temperate coliphage HK022 requires integration host factor (IHF) for lytic growth. The determinant responsible for this requirement was identified as a new gene (vol) located between genes P and Q. This gene encodes a DNA-binding protein (Roi) containing a helix-turn-helix motif. We have shown that Roi binds a site within its own gene that is closely linked to an IHF binding site. By gel retardation experiments, we have found that IHF binding stabilizes the interaction of Roi with its gene. We have isolated three independent phage mutants that are able to grow on an IHF- host. They carry different mutations scattered in the roi gene and specifying single amino-acid changes. The interactions of all three Roi mutant proteins with the Roi binding site differed from that of the wild type. Roi displays strong similarities, in its C-terminal half, to two putative DNA-binding proteins of bacteriophage P1:Ant1 and KilA. The mode of action of the Roi protein and the possibility that IHF is modulating the expression and/or the action of Roi are discussed.
引用
收藏
页码:4077 / 4083
页数:7
相关论文
共 31 条
[1]   ESCHERICHIA-COLI INTEGRATION HOST FACTOR STABILIZES BACTERIOPHAGE MU-REPRESSOR INTERACTIONS WITH OPERATOR DNA INVITRO [J].
ALAZARD, R ;
BETERMIER, M ;
CHANDLER, M .
MOLECULAR MICROBIOLOGY, 1992, 6 (12) :1707-1714
[2]   THE ESCHERICHIA-COLI-RPOB60 MUTATION BLOCKS ANTITERMINATION BY COLIPHAGE HK022 Q-FUNCTION [J].
ATKINSON, BL ;
GOTTESMAN, ME .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (01) :29-37
[3]   DETERMINANTS OF CORRECT RES SITE ALIGNMENT IN SITE-SPECIFIC RECOMBINATION BY TN3 RESOLVASE [J].
BEDNARZ, AL ;
BOOCOCK, MR ;
SHERRATT, DJ .
GENES & DEVELOPMENT, 1990, 4 (12B) :2366-2375
[4]   THE ESCHERICHIA-COLI PROTEIN, FIS - SPECIFIC BINDING TO THE ENDS OF PHAGE MU DNA AND MODULATION OF PHAGE-GROWTH [J].
BETERMIER, M ;
LEFRERE, V ;
KOCH, C ;
ALAZARD, R ;
CHANDLER, M .
MOLECULAR MICROBIOLOGY, 1989, 3 (04) :459-468
[5]  
BUJARD H, 1987, METHOD ENZYMOL, V155, P416
[6]   COMPARATIVE MOLECULAR-BIOLOGY OF LAMBDOID PHAGES [J].
CAMPBELL, A .
ANNUAL REVIEW OF MICROBIOLOGY, 1994, 48 :193-222
[7]   A ZINC-BINDING REGION IN THE BETA' SUBUNIT OF RNA-POLYMERASE IS INVOLVED IN ANTITERMINATION OF EARLY TRANSCRIPTION OF PHAGE HK022 [J].
CLERGET, M ;
JIN, DJ ;
WEISBERG, RA .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (04) :768-780
[8]   GROWTH-PHASE VARIATION OF INTEGRATION HOST FACTOR LEVEL IN ESCHERICHIA-COLI [J].
DITTO, MD ;
ROBERTS, D ;
WEISBERG, RA .
JOURNAL OF BACTERIOLOGY, 1994, 176 (12) :3738-3748
[9]   THE ROLE OF INTEGRATION HOST FACTOR IN GENE-EXPRESSION IN ESCHERICHIA-COLI [J].
FREUNDLICH, M ;
RAMANI, N ;
MATHEW, E ;
SIRKO, A ;
TSUI, P .
MOLECULAR MICROBIOLOGY, 1992, 6 (18) :2557-2563
[10]   INTEGRATION HOST FACTOR - A PROTEIN FOR ALL REASONS [J].
FRIEDMAN, DI .
CELL, 1988, 55 (04) :545-554