Isolation and characterization of microsatellites in Echinochloa (L.) Beauv. spp.

被引：14

作者：

Danquah, EY

Hanley, SJ

Brookes, RC

Aldam, C

Karp, A ^{[1
]}

机构：

[1] Univ Bristol, Dept Agr Sci, IACR Long Ashton Res Stn, Bristol BS41 9AF, Avon, England

[2] Univ Ghana, Dept Crop Sci, Legon, Ghana

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 01期

关键词：

Echinochloa; microsatellites; polymorphism; weeds;

D O I：

10.1046/j.1471-8286.2002.00144.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five primer pairs were developed that amplify microsatellite loci in three agronomically important Echinochloa (L.) Beauv. species: E. colona (L.) Link, E. crus-galli (L.) Beauv. and E. crus-pavonis (Kunth) Schultes. The microsatellites were tested on 24 individuals representing three species collected in rice fields from different geographical regions and revealed 3-7 alleles per microsatellite. Gene diversity [1-Sigmap(ij)(2)] for four polymorphic loci within E. crus-galli ranged from 0.12 to 0.61. Alleles at a fifth locus were useful in discriminating the species. The microsatellites should provide useful markers for intraspecific diversity studies and aid classification of species within this complex genus.

引用

页码：54 / 56

页数：3

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