Mitochondrial histone-like DNA-binding proteins are essential for normal cell growth and mitochondrial function in Crithidia fasciculata

被引:25
作者
Avliyakulov, NK
Lukes, J
Ray, DS
机构
[1] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA
[3] Acad Sci Czech Republ, Inst Parasitol, CR-37005 Ceske Budejovice, Czech Republic
[4] Univ S Bohemia, Fac Biol, Ceske Budejovice 37005, Czech Republic
关键词
D O I
10.1128/EC.3.2.518-526.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Crithidia fasciculata KAP2 and KAP3 proteins are closely related kinetoplast-specific histone-like DNA-binding proteins. The KAP2 and KAP3 genes are 46% identical and are arranged in tandem on the chromosomal DNA. Disruption of both alleles of either gene alone shows no detectable phenotype. However, replacement of both copies of the sequence encoding the entire KAP2 and KAP3 locus increases maxicircle mRNA levels two- to fourfold. These double-knockout cells are viable but grow extremely slowly, have reduced respiration and very abnormal cell morphologies, and accumulate numerous large vacuoles. The extreme phenotype of these mutant cells suggests an important role for the KAP2 and KAP3 proteins in mitochondrial metabolism and cell growth.
引用
收藏
页码:518 / 526
页数:9
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