Identification of Cellulase Genes from the Metagenomes of Compost Soils and Functional Characterization of One Novel Endoglucanase

被引：69

作者：

Pang, Hao ^{[1
,2
]}

Zhang, Peng ^{[1
,2
]}

Duan, Cheng-Jie ^{[1
,2
]}

Mo, Xin-Chun ^{[1
,2
]}

Tang, Ji-Liang ^{[1
,2
]}

Feng, Jia-Xun ^{[1
,2
]}

机构：

[1] Guangxi Univ, Coll Life Sci & Technol, Minist Educ Microbial & Plant Genet Engn, Guangxi Key Lab Subtrop Bioresources Conservat &, Nanning 530005, Guangxi, Peoples R China

[2] Guangxi Key Lab BioInd Technol, Nanning 530004, Guangxi, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2009年 / 58卷 / 04期

基金：

中国国家自然科学基金;

关键词：

UNCULTURED MICROORGANISMS; ENZYMES; CLONING; BIOTECHNOLOGY; BACTERIA; SEQUENCE;

D O I：

10.1007/s00284-008-9346-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Metagenomics, a new research field developed over the past decade, aims to identify potential enzymes from nonculturable microbes. In this study, genes encoding three glycoside hydrolase family (GHF) 9 endoglucanases and one GHF 5 endoglucanase were cloned and identified from the metagenome of the compost soils. The shared identities between the predicted amino acid sequences of these genes and their closest homologues in the database were less than 70%. One GHF 9 endoglucanase, Umcel9B, was further characterized. The recombinant protein, Umcel9B, showed activity against carboxymethyl cellulose, indicating that Umcel9B is an endoactive enzyme. Enzymatic activity occurs optimally at a pH of 7.0 and a temperature of 25A degrees C.

引用

页码：404 / 408

页数：5

共 21 条

[1]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[2] Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans [J].