Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles: Synthesis, characterization, and application to MRI contrast agents

被引：25

作者：

Ahmad, Md. Wasi ^{[1
]}

Kim, Cho Rong ^{[1
]}

Baeck, Jong Su ^{[2
]}

Chang, Yongmin ^{[2
,4
]}

Kim, Tae Jeong ^{[3
,4
]}

Bae, Ji Eun ^{[4
]}

Chaed, Kwon Seok ^{[4
,5
]}

Lee, Gang Ho ^{[1
,4
]}

机构：

[1] Kyungpook Natl Univ, Coll Nat Sci, Dept Chem, Taegu 702701, South Korea

[2] KNU, Dept Mol Med & Med Biol Engn, Sch Med, Taegu 702701, South Korea

[3] KNU, Coll Engn, Dept Appl Chem, Taegu 702701, South Korea

[4] KNU, Dept Nanosci & Nanotechnol, Taegu 702701, South Korea

[5] KNU, Teachers Coll, Dept Biol Educ, Taegu 702701, South Korea

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2014年 / 450卷

基金：

新加坡国家研究基金会;

关键词：

BSA; C-BSA; Ultrasmall Gd2O3 nanoparticle; Nanoparticle carrier; MRI; Contrast agent; GADOLINIUM OXIDE NANOPARTICLES; THERAPEUTIC AGENTS; IN-VITRO; ATR-FTIR; GD-DTPA; SURFACE; ACID; COMPLEXATION; RECOGNITION; SEQUENCE;

D O I：

10.1016/j.colsurfa.2014.03.011

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

Bovine serum albumin (BSA) (M-n = 66.5 kD, size =14 x 4 x 4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core d(avg) = 2.0 nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1 =6.0s(-1) mM(-1) and r2 =28.0 s(-1) mM(-1) for BSA-PEGD-GNPs and r1 =7.6 s(-1) mM(-1) and r2 =22.0s(-1) mM(-1) for C-BSA-PEGDGNPs). Three tesla T-2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：67 / 75

页数：9

共 36 条

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