Identification and quantification of Tuber melanosporum Vitt sterols

被引：27

作者：

Harki, E

Klaebe, A

Talou, T

Dargent, R

机构：

[1] ECOLE NATL SUPER CHIM,LAB CHIM AGROIND,F-31077 TOULOUSE,FRANCE

[2] UNIV TOULOUSE 3,LAB IMRCP,URA CNRS 470,GRP CHIM ORGAN BIOL,F-31062 TOULOUSE,FRANCE

[3] UNIV TOULOUSE 3,LAB MYCOL,F-31062 TOULOUSE,FRANCE

来源：

STEROIDS | 1996年 / 61卷 / 10期

关键词：

sterols; Tuber melanosporum; truffle; ergosterol; brassicasterol;

D O I：

10.1016/S0039-128X(96)00121-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sterol composition of Tuber melanosporum was examined by medium-pressure liquid and high-performance liquid chromatography nuclear magnetic resonance spectroscopy, and mass spectroscopy. Ergosterol (ergosta-5,7,22-trienol) and brassicasterol (ergosta-5,22-dienol) were identified as the major components (90%). Their quantities and their relative concentrations, probably good indicators for differentiating mature truffles from immature colored ones, were determined during the maturation of the fungus. The results show that the total quantity of sterols decreased with the age of the fungus, but that the ergosterol/brassicasterol ratio remained relatively constant. (C) 1996 by Elsevier Science Inc.

引用

页码：609 / 612

页数：4

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