Biochemical properties of skin collagens isolated from black carp (Mylopharyngodon piceus)

被引：42

作者：

Jia, Yuanjun ^{[2
]}

Wang, Haibo ^{[1
]}

Wang, Haiyin ^{[3
]}

Li, Yunyan ^{[1
]}

Wang, Min ^{[1
]}

Zhou, Jian ^{[2
]}

机构：

[1] Wuhan Polytech Univ, Dept Chem & Environm Engn, Wuhan, Hubei, Peoples R China

[2] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China

[3] Huzhong Univ Sci & Technol, Tongji Med Collage, Wuhan, Hubei, Peoples R China

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2012年 / 21卷 / 06期

基金：

中国国家自然科学基金;

关键词：

collagen; skin; black carp; biochemical property; biocompatibility; ACID-SOLUBLE COLLAGEN; I COLLAGEN; BONE; NILOTICUS; CHITOSAN; SCALE; GELS;

D O I：

10.1007/s10068-012-0211-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted with the yield of 15.5 and 26.5% on the basis of dry weight from the skin of black carp (Mylopharyngodon piceus). ASC and PSC were similar in amino compositions and subunit compositions, but they were slightly different in the secondary structure. Both of ASC and PSC, either rehydrated in distilled water or in acetic acid, had a lower denaturation temperature than pig skin collagen (PPSC), but their denaturation temperature were higher than that of collagens from other freshwater fish. Intrinsic viscosity of different collagens decreased in the order of PPSC > PSC > ASC. Both of ASC and PSC showed only partial degradation in collagenase solution, but they revealed more sensitive to collagenase compared with PPSC. Black carp skin collagens did not induce a significant cytotoxic effect according to the results of in vitro cytotoxicity test.

引用

页码：1585 / 1592

页数：8

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