Differences in steroid 5α-reductase iso-enzymes expression between normal and pathological human prostate tissue

被引:83
作者
Iehlé, C
Radvanyi, F
de Medina, SGD
Ouafik, L
Gérard, H
Chopin, D
Raynaud, JP
Martin, PM
机构
[1] Univ Paris 06, DRI, F-75252 Paris 05, France
[2] Fac Med Nord, INSERM CJF 9311, Lab Cancerol Expt, F-13916 Marseille, France
[3] Inst Curie, UMR 144, F-75248 Paris 05, France
[4] Fac Med Nord, INSERM U297, F-13916 Marseille 20, France
[5] Hop Henri Mondor, Serv Urol, F-94010 Creteil, France
关键词
5; alpha-reductase; prostate; stroma; epithelium; ISH; RT-PCR;
D O I
10.1016/S0960-0760(99)00030-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied the expression level and cell-specific expression patterns of 5 alpha-reductase (5 alpha-R) types 1 and 2 iso-enzymes in human hyperplastic and malignant prostate tissue by semi-quantitative RT-PCR and in situ hybridisation analyses. In situ hybridisation established that 5 alpha-R1 mRNA is preferentially expressed by epithelial cells and little expressed by stromal cells whereas 5 alpha-R2 mRNA is expressed by both epithelium and stroma. Semi-quantitative RT-PCR has been performed on total RNA from different zones of normal prostate, BPH tissues and liver. We found that 5 alpha-R1 and 5 alpha-R2 mRNAs expression was near the same in all zones of normal prostate. In BPH tissue, 5 alpha-R1 and 5 alpha-R2 mRNAs expression was slightly but significantly increased, when it was compared to the levels recorded for normal prostate. In cancer samples, 5 alpha-R1 mRNA expression was higher than in normal and hyperplastic prostate but the level of 5 alpha-R2 mRNA was not statistically different from that observed in the different zones of normal prostate. In liver, 5 alpha-R2 mRNA level was similar to that measured in BPH but 5 alpha-R1 mRNA expression was ten times higher. The increase observed in 5 alpha-R isoenzymes expression in BPH tissue could play an important role in the pathogenesis and/or maintenance of the disease. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:189 / 195
页数:7
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