Interference-free sample preparation for the determination of plasma oxalate analyzed by HPLC-ER:: preliminary results from calcium oxalate stone-formers and non-stone-formers

被引:19
作者
Hönow, R [1 ]
Simon, A [1 ]
Hesse, A [1 ]
机构
[1] Univ Bonn, Klin & Poliklin Urol, Dept Urol, Div Expt Urol, D-53105 Bonn, Germany
关键词
oxalate generation; ascorbate; sample preparation; protein binding; sex-related; age-related;
D O I
10.1016/S0009-8981(01)00729-X
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Oxalate generation at pH-values above 5.0 and an oxalate-protein binding in acidified plasma would appear to complicate (lie determination of oxalate in plasma. Methods: To avoid complex sample preparation we used a high-performance liquid chromatographic system with an inline enzyme reactor (HPLC-ER) containing immobilised oxalate oxidase. The detection limit was 0.68 mumol/l. Blood was drawn in lithium-heparin vessels and immediately centrifuged at 4 T. The yielded plasma was ultrafiltered using a Centrisart-I-tube. To inhibit oxalate generation by ascorbic acid, the ultrafiltrate was acidified with 1 mol/l hydrochloric acid during ultrafiltration at 4 T. The liquid thus yielded was used for HPLC-ER analysis. Blood samples were obtained from 133 healthy adults (63 men, 70 women, aged 20-94 years) with no history of renal disorder and from 79 patients (53 men, 26 women, aged 19-77 years) with a history of calcium oxalate stone formation. Results: Mean plasma oxalate was 2.65 +/- 2.31 mumol/l for healthy subjects and 4.21 +/- 0.56 mumol/l for stone formers. Conclusions: Analysis yielded no significant differences between males and females. A correlation between age and plasma oxalate was found for the healthy adults (p < 0.001). (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:19 / 24
页数:6
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