An improved method for preparing the chromosomes of pines and other gymnosperms

被引：11

作者：

Murray, BG

Davies, BJ

机构：

[1] School of Biological Sciences, University of Auckland, Auckland

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1996年 / 71卷 / 03期

关键词：

cellulase; chromosome spreading; enzyme digestion; gymnosperms; pectinase;

D O I：

10.3109/10520299609117146

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple technic is described to produce wall spread gymnosperm chromosomes. Root tip meristems are digested with a pectinase:cellulase mixture to produce a cell suspension which then is squashed to yield hat, well spread chromosome complements that can be stained or used for in situ hybridization.

引用

页码：115 / 117

页数：3

共 13 条

[1] FEULGEN CYTOPHOTOMETRY OF PINE NUCLEI .2. EFFECT OF PECTINASE USED IN CELL-SEPARATION
BERLYN, GP
DHILLON, SS
MIKSCHE, JP
[J]. STAIN TECHNOLOGY, 1979, 54 (04): : 201 - 204
[2] KARYOTYPE OF SLASH PINE (PINUS-ELLIOTTII VAR ELLIOTTII) USING PATTERNS OF FLUORESCENCE IN-SITU HYBRIDIZATION AND FLUOROCHROME BANDING
DOUDRICK, RL
HESLOPHARRISON, JS
NELSON, CD
SCHMIDT, T
NANCE, WL
SCHWARZACHER, T
[J]. JOURNAL OF HEREDITY, 1995, 86 (04) : 289 - 296
[3] GEBER G, 1988, PLANT SYST EVOL, V158, P97, DOI 10.1007/BF00936336
[4] Hizume M, 1988, MEM FAC ED EHIME U N, V8, P1
[5] KARYOTYPE ANALYSIS AND THE DISTRIBUTION OF CONSTITUTIVE HETEROCHROMATIN IN 5 SPECIES OF PINUS
MACPHERSON, P
FILION, WG
[J]. JOURNAL OF HEREDITY, 1981, 72 (03) : 193 - 198
[6] GENOMIC CONSTANCY DURING THE DEVELOPMENT OF LATHYRUS-ODORATUS CULTIVARS
MURRAY, BG
HAMMETT, KRW
STANDRING, LS
[J]. HEREDITY, 1992, 68 : 321 - 327
[7] HIGHLY EFFECTIVE CELL SYNCHRONIZATION IN PLANT-ROOTS BY HYDROXYUREA AND AMIPROPHOS-METHYL OR COLCHICINE
PAN, WH
HOUBEN, A
SCHLEGEL, R
[J]. GENOME, 1993, 36 (02) : 387 - 390
[8] PEDERICK L. A., 1967, SILVAE GENET, V16, P69
[9] PRINGLE GJ, 1993, BOT J LINN SOC, V111, P331, DOI 10.1111/j.1095-8339.1993.tb01907.x
[10] SAX KARL, 1933, JOUR ARNOLD ARBORETUM, V14, P356

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