Isolation from beer and structural determination of a potent stimulant of gastrin release

被引:24
作者
Yokoo, Y
Kohda, H
Kusumoto, A
Naoki, H
Matsumoto, N
Amachi, T
Suwa, Y
Fukazawa, H
Ishida, H
Tsuji, K
Nukaya, H
机构
[1] Suntory Ltd, Hlth Sci Lab, Shimamoto, Osaka 6188503, Japan
[2] Suntory Ltd, Tech Dev Dept, Shimamoto, Osaka 6188503, Japan
[3] Suntory Ltd, Tech Dev Dept, Shimamoto, Osaka 6180001, Japan
[4] Suntory Ltd, Hlth Sci Lab, Shimamoto, Osaka 6180001, Japan
[5] Suntory Inst Bioorgan Res, Shimamoto, Osaka 6188503, Japan
[6] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan
来源
ALCOHOL AND ALCOHOLISM | 1999年 / 34卷 / 02期
关键词
D O I
10.1093/alcalc/34.2.161
中图分类号
R194 [卫生标准、卫生检查、医药管理];
学科分类号
摘要
Beer was subjected to five successive chromatographic procedures to isolate the gastrin release-inducing activity, guided by bioassay of the fractions in anaesthetized Donryu rats. The procedures were: (1) hydrophobic interaction chromatography (aqueous effluent with an HP20 column); (2) weak cation-exchange chromatography (1 M acetic acid eluate with a CM Sephadex C-25 column); (3) gel filtration (methanol eluate with a Sephadex LH-20 column); (4) same as (2); (5) high-performance liquid chromatography (YMC-Pack ODS-AM with 7% acetonitrile-0.01 M HCl). The active component finally isolated had a specific activity similar to 10 000 times higher than that of beer. It was identified by means of mass, H-1- and C-13-nuclear magnetic resonance spectral analyses as N-methyltyramine (NMT). The dose of NMT giving maximal gastrin-releasing activity was 25 mu g/kg, and the 50% effective dose was similar to 10 mu g/kg on oral administration to rats. NMT was isolated and identified as a gastrin release inducer in beer. Its concentration in beer is sufficient to account for most of the activity of beer.
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页码:161 / 168
页数:8
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