Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica

被引：122

作者：

Bernal, D

de la Rubia, JE

Carrasco-Abad, AM

Toledo, R

Mas-Coma, S

Marcilla, A

机构：

[1] Univ Valencia, Fac Farm, Dept Parasitol & Biol Celular, E-46100 Valencia, Spain

[2] Univ Valencia, Fac Ciencies, Dept Bioquim & Biol Mol, E-46100 Valencia, Spain

来源：

FEBS LETTERS | 2004年 / 563卷 / 1-3期

关键词：

excretory-secretory; enolase; plasminogen binding; Fasciola hepatica;

D O I：

10.1016/S0014-5793(04)00306-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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页码：203 / 206

页数：4

相关论文

共 33 条

[1] Linking proteome and genome: how to identify parasite proteins [J].

Ashton, PD ;

Curwen, RS ;

Wilson, RA .

TRENDS IN PARASITOLOGY, 2001, 17 (04) :198-202

[2]

Behm CA, 1999, FASCIOLOSIS, P185

[3] Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components [J].

Berasain, P ;

Goni, F ;

McGonigle, S ;

Dowd, A ;

Dalton, JP ;

Frangione, B ;

Carmona, C .

JOURNAL OF PARASITOLOGY, 1997, 83 (01) :1-5

[4] Fasciola hepatica:: Parasite-secreted proteinases degrade all human IgG subclasses:: Determination of the specific cleavage sites and identification of the immunoglobulin fragments produced [J].

Berasain, P ;

Carmona, C ;

Frangione, B ;

Dalton, JP ;

Goñi, F .

EXPERIMENTAL PARASITOLOGY, 2000, 94 (02) :99-110

[5] Identification of a novel plasmin(ogen)-binding motif in surface displayed α-enolase of Streptococcus pneumoniae [J].

Bergmann, S ;

Wild, D ;

Diekmann, O ;

Frank, R ;

Bracht, D ;

Chhatwal, GS ;

Hammerschmidt, S .

MOLECULAR MICROBIOLOGY, 2003, 49 (02) :411-423

[6] α-Enolase of Streptococcus pneumoniae is a plasmin(ogen)-binding protein displayed on the bacterial cell surface [J].

Bergmann, S ;

Rohde, M ;

Chhatwal, GS ;

Hammerschmidt, S .

MOLECULAR MICROBIOLOGY, 2001, 40 (06) :1273-1287

[7] Potential role for excretory-secretory forms of glutathione-S-transferase (GST) in Fasciola hepatica [J].

Cervi, L ;

Rossi, G ;

Masih, DT .

PARASITOLOGY, 1999, 119 :627-633

[8] Characterization of two cysteine proteinases secreted by Fasciola hepatica and demonstration of their kininogenase activity [J].

Cordova, M ;

Jara, J ;

Del Nery, E ;

Hirata, IY ;

Araújo, MS ;

Carmona, AK ;

Juliano, MA ;

Juliano, L .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 116 (02) :109-115

[9] THIOL PROTEASES RELEASED INVITRO BY FASCIOLA-HEPATICA [J].

DALTON, JP ;

HEFFERNAN, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 35 (02) :161-166

[10] Fasciola hepatica cathepsin L-like proteases:: biology, function, and potential in the development of first generation liver fluke vaccines [J].

Dalton, JP ;

Neill, SO ;

Stack, C ;

Collins, P ;

Walshe, A ;

Sekiya, M ;

Doyle, S ;

Mulcahy, G ;

Hoyle, D ;

Khaznadji, E ;

Moiré, N ;

Brennan, G ;

Mousley, A ;

Kreshchenko, N ;

Maule, AG ;

Donnelly, SM .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2003, 33 (11) :1173-1181

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