Evidence that two genomic species of Rhizobium are associated with Medicago truncatula

被引：31

作者：

Rome, S

Brunel, B

Normand, P

Fernandez, M

CleyetMarel, JC

机构：

[1] ECOLE NATL SUPER AGRON MONTIPELLIER,INRA,LAB RECH SYMBIOTES RACINES,F-34060 MONTPELLIER,FRANCE

[2] ECOLE NATL SUPER AGRON MONTIPELLIER,INRA,UNITE FORMAT & RECH SCI SOL,F-34060 MONTPELLIER 01,FRANCE

来源：

ARCHIVES OF MICROBIOLOGY | 1996年 / 165卷 / 04期

关键词：

Rhizobium meliloti; Medicago truncatula; PCR/RFLP; 16S rDNA; nifD-K region; nodD1; gene; nodD2; DNA/DNA hybridization;

D O I：

10.1007/s002030050328

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization.

引用

页码：285 / 288

页数：4

共 17 条

[1]

[Anonymous], INT BIOL PROGRAM HDB

[2]

Baker DG, 1990, PLANT MOL BIOL REP, V8, P40

[3] ESCHERICHIA-VULNERIS - A NEW SPECIES OF ENTEROBACTERIACEAE ASSOCIATED WITH HUMAN WOUNDS [J].