The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus

被引:44
作者
Endo, M
Matsubara, H
Kokubun, T
Masuko, H
Takahata, Y
Tsuchiya, T
Fukuda, H
Demura, T
Watanabe, M
机构
[1] Iwate Univ, Fac Agr, Lab Plant Breeding, Morioka, Iwate 0208550, Japan
[2] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan
[3] Univ Tokyo, Dept Biol Sci, Grad Sch Sci, Tokyo 1130033, Japan
[4] Inst Phys & Chem Res, Plant Sci Ctr, Wako, Saitama 3510198, Japan
关键词
cDNA microarray; Anther-specific gene; Pistil-specific gene; reverse transcriptase polymerase chain reaction; Lotus japonicus;
D O I
10.1016/S0014-5793(02)02371-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:229 / 237
页数:9
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