Cloning, tissue distribution, genomic organization, and functional characterization of NBCS, a new member of the sodium bicarbonate cotransporter family

被引：160

作者：

Pushkin, A ^{[1
]}

Abuladze, N ^{[1
]}

Lee, I ^{[1
]}

Newman, D ^{[1
]}

Hwang, J ^{[1
]}

Kurtz, I ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Div Nephrol, Ctr Hlth Sci, Sch Med, Los Angeles, CA 90095 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 23期

关键词：

D O I：

10.1074/jbc.274.23.16569

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous functional studies have demonstrated that muscle intracellular pH regulation is mediated by sodium-coupled bicarbonate transport, Na+/H+ exchange, and Cl-/bicarbonate exchange. We report the cloning, sequence analysis, tissue distribution, genomic organization, and functional analysis of a new member of the sodium bicarbonate cotransporter (NBC) family, NBC3, from human skeletal muscle. mNBC3 encodes a 1214-residue polypeptide with 12 putative membrane-spanning domains. The similar to 7.8-kilobase transcript is expressed uniquely in skeletal muscle and heart. The NBCS gene (SLC4A7) spans similar to 80 kb and is composed of 25 coding exons and 24 introns that are flanked by typical splice donor and acceptor sequences. Expression of mNBC3 cRNA in Xenopus laevis oocytes demonstrated that the protein encodes a novel stilbene-insensitive 5-(N-ethyl-N-isopropyl)-amiloride-inhibitable sodium bicarbonate cotransporter.

引用

页码：16569 / 16575

页数：7

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