Accumulation of phthalides in elicitor-treated cell suspension cultures of Petroselinum crispum

被引:26
作者
Hagemeier, J
Batz, O
Schmidt, J
Wray, V
Hahlbrock, K
Strack, D
机构
[1] Inst Pflanzenbiochem, Abt Sekundarstoffwechsel, D-06120 Halle, Saale, Germany
[2] Max Planck Inst Zuchtungsforsch, Biochem Abt, D-50829 Cologne, Germany
[3] Inst Pflanzenbiochem, Abt Nat Stoffchem, D-06120 Halle, Saale, Germany
[4] Gesell Biotechnol Forsch mbH, D-38124 Braunschweig, Germany
关键词
Petroselinum crispum; Apiaceae; cell culture; elicitation; butylidenephthalides;
D O I
10.1016/S0031-9422(99)00072-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study describes the effect of a Phytophthora sojae 25-amino acid oligopeptide (Pep25) elicitor on the secondary metabolism of parsley cell cultures (Petroselinum crispum L.). HPLC analysis of the accumulated compounds in the elicitor-treated cultures revealed the expected accumulation of furanocoumarins (e.g. marmesin and bergapten) as well as various non-coumarin compounds which have not been described previously to occur in this cell culture. These compounds were isolated by preparative HPLC and identified by spectroscopic methods (MS, NMR) as 5-hydroxy- and 7-hydroxy-3-butylidenephthalides including two novel conjugates of the 7-hydroxy derivative, i.e. 7-O-glucoside and 7-O-(6'-malonylglucoside). (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:629 / 635
页数:7
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