Expression analysis of new Ly49 genes:: most transcripts of Ly49j lack the transmembrane domain

被引:32
作者
McQueen, KL
Lohwasser, S
Takei, F
Mager, DL
机构
[1] Univ British Columbia, Terry Fox Lab, British Columbia Canc Agcy, Vancouver, BC V5Z 1L3, Canada
[2] Univ British Columbia, Dept Med Genet, Vancouver, BC V5Z 1L3, Canada
[3] Univ British Columbia, Dept Pathol, Vancouver, BC V5Z 1L3, Canada
基金
英国医学研究理事会; 加拿大自然科学与工程研究理事会;
关键词
NK cell; inhibitory receptor; Ly49; alternative splicing;
D O I
10.1007/s002510050665
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Five new Ly49 genes, named Ly49j-n, have recently been identified in C57BL/6 mice. This study examined the expression of three of these new genes, Ly49j, k, and n. To determine whether the Ly49j, k, and n genes were transcribed, gene-specific primers were used to amplify cDNA clones for each gene from C57BL/6 interleukin-2-activated natural killer (NK) cell cDNA. A full-length cDNA for Ly49j was detected which encodes a 267 amino acid protein and shares approximately 96% nucleotide identity with Ly49c and i. COS cells transfected with the Ly49j cDNA were shown to react with the monoclonal antibody 8H7, suggesting that the gene likely encodes a functional protein. Many different sized Ly49k and n transcripts were observed, although it is likely that they do not encode functional proteins due to missing exons or severe truncations in the open reading frames. Interestingly, the most abundant Ly49j transcript detected was shown to lack exon 3, which encodes the transmembrane domain. Similar studies performed on the same source of NK cell cDNA using Ly49c- and i-specific primers revealed the presence of transmembrane-less Ly49i transcripts, although at a much lower frequency than observed for Ly49j. We also detected Ly49g and h transcripts lacking the transmembrane domain. Despite the absence of the transmembrane region, the resulting Ly49 transcripts maintain their open reading frames, and therefore could potentially encode cytoplasmic proteins with a role in NK cell function.
引用
收藏
页码:685 / 691
页数:7
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