Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans

被引：26

作者：

Bertuzzi, F

Zacchetti, D

Berra, C

Socci, C

Pozza, G

Pontiroli, AE

Grohovaz, F

机构：

[1] CNR,CELLULAR & MOLEC PHARMACOL CTR,I-20132 MILAN,ITALY

[2] IST SCI SAN RAFFAELE,DIBIT,B CECCARELLI CTR,I-20132 MILAN,ITALY

[3] IST SCI SAN RAFFAELE,DEPT SURG,I-20132 MILAN,ITALY

[4] IST SCI SAN RAFFAELE,DIBIT,DEPT MED,I-20132 MILAN,ITALY

来源：

FEBS LETTERS | 1996年 / 379卷 / 01期

关键词：

Islets of Langerhans; Fura-2; Ca2+ oscillation; Ca2+ wave; insulin secretion;

D O I：

10.1016/0014-5793(95)01422-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca2+](i), in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca2+](i) changes in few cells. After ten days, insulin response was partially restored and [Ca2+](i) recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca2+](i) variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.

引用

页码：21 / 25

页数：5

共 19 条

[1]

BERGSTEN P, 1994, J BIOL CHEM, V269, P8749

[2] THE GAP JUNCTION - A CHANNEL FOR MULTIPLE FUNCTIONS [J].