Urethane dimethacrylate induces cytotoxicity and regulates cyclooxygenase-2, hemeoxygenase and carboxylesterase expression in human dental pulp cells

被引：38

作者：

Chang, Hsiao-Hua ^{[1
,2
,3
]}

Chang, Mei-Chi ^{[4
]}

Wang, Hsin-Hui ^{[1
,2
,3
]}

Huang, Guay-Fen ^{[1
,2
,3
]}

Lee, Yuan-Ling ^{[1
,2
,3
]}

Wang, Yin-Lin ^{[1
,2
,3
]}

Chan, Chiu-Po ^{[5
]}

Yeung, Sin-Yuet ^{[4
]}

Tseng, Shuei-Kuen ^{[1
,2
,3
]}

Jeng, Jiiang-Huei ^{[1
,2
,3
]}

机构：

[1] Natl Taiwan Univ Hosp, Dept Dent, Lab Pharmacol Toxicol & Pulp Biol, Taipei, Taiwan

[2] Natl Taiwan Univ Hosp, Sch Dent, Taipei, Taiwan

[3] Natl Taiwan Univ, Coll Med, Taipei 10764, Taiwan

[4] Chang Gung Univ Sci & Technol, Biomed Sci Team, Tao Yuan, Taiwan

[5] Chang Gung Mem Hosp, Dept Dent, Taipei 10591, Taiwan

来源：

ACTA BIOMATERIALIA | 2014年 / 10卷 / 02期

关键词：

Dental pulp; Inflammation; Prostanoids; Resin monomers; Urethane dimethacrylate; TRIETHYLENE GLYCOL DIMETHACRYLATE; 2-HYDROXYETHYL METHACRYLATE; N-ACETYLCYSTEINE; EPITHELIAL-CELLS; MAMMALIAN-CELLS; RESIN MONOMERS; MESSENGER-RNA; CYCLE; MECHANISMS; BISGMA;

D O I：

10.1016/j.actbio.2013.10.006

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The toxic effect of urethane dimethacrylate (UDMA), a major dental resin monomer, on human dental pulp is not fully clear. In this study, we investigated the influence of UDMA on the cytotoxicity, cell cycle distribution, apoptosis and related gene expression of dental pulp cells. The role of reactive oxygen species, hemeoxygenase-1 (HO-1) and carboxylesterase (CES) in UDMA cytotoxicity, was evaluated. UDMA induced morphological changes of pulp cells and decreased cell viability by 29-49% at concentrations of 0.1-0.35 mM. UDMA induced G0/G1, G2/M cell cycle arrest and apoptosis. The expression of cdc2, cyclinB1 and cdc25C was inhibited by UDMA. Moreover, UDMA stimulated COX-2, HO-1 and CES2 mRNA expression of pulp cells. The cytotoxicity of UDMA was attenuated by N-acetyl-L-cysteine, catalase and esterase, but was enhanced by Zn-protoporphyrin (HO-1 inhibitor), BNPP (CES inhibitor) and loperamide (CES2 inhibitor). Exposure of UDMA may potentially induce the inflammation and toxicity of dental pulp. These findings are important for understanding the clinical response of human pulp to resin monomers after operative restoration and pulp capping, and also provide clues for improvement of dental materials. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：722 / 731

页数：10

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