Changes in sensitivity of a human myeloid cell line (U937) to metal toxicity after glutathione depletion.

被引:7
作者
Danfour, M [1 ]
Schorah, CJ [1 ]
Evans, SW [1 ]
机构
[1] Univ Leeds, Res Sch Med, Mol Epidemiol Unit, Leeds LS2 9JT, W Yorkshire, England
关键词
D O I
10.3109/08923979909052763
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Glutathione (GSH) concentrations have been determined in the human myeloid cell line U937. The effects of modulating GSH concentration on sensitivity to metal toxicity has also been examined. Intracellular concentrations of GSH increased as the cells entered into the cell cycle, reaching a maximum level after 24 hours of cell culture, after which levels declined. Cell concentration was also observed to influence intracellular GSH concentrations. A reciprocal relationship was observed with higher maximum intracellular GSH concentrations being measured in cultures initiated with smaller cell number. The relative toxicity's determined for five metal chlorides were mercury > cadmium > cobalt > zinc > gold. Treatment of cells with N-acetylcysteine (NAC) increased intracellular GSH but had little effect on the absolute or relative toxicity's of the metals. Treatment of cells with L-buthionine-(S-R)-sulfoximine (BSO) depleted intracellular GSH and resulted in increased sensitivity of the cells to gold, 40 fold, cadmium 8 fold and mercury 3 fold.
引用
收藏
页码:277 / 293
页数:17
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