Structure of a selectin-like mutant of mannose-binding protein complexed with sialylated and sulfated Lewis(x) oligosaccharides

被引:82
作者
Ng, KKS [1 ]
Weis, WI [1 ]
机构
[1] STANFORD UNIV,SCH MED,DEPT BIOL STRUCT,STANFORD,CA 94305
关键词
D O I
10.1021/bi962564e
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rat serum mannose-binding protein in which residues 211-213 have been changed to the Lys-Lys-Lys sequence found in E-selectin binds HL-60 cells and the oligosaccharide 3'-NeuAc-Le(x). To understand how this mutant, designated K3, mimics the carbohydrate-binding properties of E-selectin, structures of K3 alone and in complexes with 3'-NeuAc-Le(x), 3'-sulfo-Le(x), and 4'-sulfo-Le(x) have been determined at 1.95-2.1 Angstrom resolution by X-ray crystallography, The region of K3 that interacts with bound oligosaccharides superimposes closely with the corresponding region of unliganded E-selectin, In each of the oligosaccharide-protein complexes, the 2- and 3-OH of Fuc coordinate Ca2+ and form a network of cooperative hydrogen bonds with amino acid side chains that also coordinate the Ca2+. Lys(211) of the K3 mutant, which corresponds to Lys(111) of E-selectin, interacts with each of the three bound ligands: the N zeta atom donates a hydrogen bond to the 4-OH of Gal in 3'-NeuAc-Le(x), forms a water-mediated hydrogen bond with the 4-OH of Gal in 3'-sulfo-Le(x), and forms a salt bridge with the sulfate group of 4'-sulfo-Le(x), Lys(213) packs against an otherwise exposed aromatic residue and forms a water-mediated hydrogen bond with Lys(211) which may help to position that residue for interactions with bound oligosaccharides, These structures are consistent with previous mutagenesis and chemical modification studies which demonstrate the importance of the Ca2+ ligands as well as Lys(111) and Lys(113) for carbohydrate binding in the selectins, and they provide a structural basis for understanding the selective recognition of negatively charged Le(x) derivatives by the selectins.
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页码:979 / 988
页数:10
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