Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients

被引：38

作者：

Chen, J

Li, L

Zhang, YY

Yang, HR

Wei, YH

Zhang, L

Liu, XH

Yu, L ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

[2] Qingdao Univ, Affiliated Hosp, Yantai Yuhuangding Hosp, Dept Hematol, Yantai 264000, Shandong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 341卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Pin1; Nek6; hepatocellular carcinoma;

D O I：

10.1016/j.bbrc.2005.12.228

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The peptidyl-prolyl isomerase Pin I is prevalently overexpressed in human cancers and is regarded as a new diagnostic and therapeutic target. Pin] interacts with several proteins involved in cell cycle events in a phosphorylation-dependent manner. Among them, NIMA (never in mitosis, gene A) was first identified to interact with Pin 1. In this report, we found that Pin I could interact with Nek6, one of the human NIMA-related kinases (Neks). This interaction was confirmed by GST pull-down assay, which was further confirmed by immunoprecipitation experiments, as well as immunofluorescence colocalization. We further studied Pin I and Nek6 mRNA level in 40 pairs of hepatocellular carcinoma cases, finding significant correlations between Nek6 and Pint mRNA expression levels in these samples. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1059 / 1065

页数：7

共 19 条

[1] A mitotic cascade of NIMA family kinases - Nercc1/Nek9 activates the Nek6 and Nek7 kinases [J].