Lung endothelial barrier protection by resveratrol involves inhibition of HMGB1 release and HMGB1-induced mitochondrial oxidative damage via an Nrf2-dependent mechanism

被引:66
作者
Dong, Wen-Wen [1 ,2 ,3 ]
Liu, Yu-Jian [3 ]
Lv, Zhou [1 ,2 ]
Mao, Yan-Fei [1 ,2 ]
Wang, Ying-Wei [1 ,2 ]
Zhu, Xiao-Yan [4 ,5 ]
Jiang, Lai [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Anesthesiol, Shanghai 200092, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Surg Intens Care Unit, Shanghai 200092, Peoples R China
[3] Shanghai Univ Sport, Minist Educ, Key Lab Exercise & Hlth Sci, Sch Kinesiol, Shanghai 200438, Peoples R China
[4] Second Military Med Univ, Minist Educ, Dept Physiol, Shanghai 200433, Peoples R China
[5] Second Military Med Univ, Minist Educ, Key Lab Mol Neurobiol, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
Resveratrol; Ventilator-induced lung injury; HMGB1; Mitochondrial oxidative damage; Lung endothelial barrier; Nrf2; Free radicals; MOBILITY GROUP BOX-1; NLRP3; INFLAMMASOME; CYCLIC STRETCH; CELL-DEATH; INJURY; STRESS; KINASE; CONTRIBUTES; VENTILATION; ACTIVATION;
D O I
10.1016/j.freeradbiomed.2015.05.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
High-mobility group box 1 (HMGB1) contributes to lung vascular hyperpermeability during ventilator-induced lung injury. We aimed to determine whether the natural antioxidant resveratrol protected against HMGB1-induced endothelial hyperpermeability both in vitro and in vivo. We found that HMGB1 decreased vascular endothelial (VE)-cadherin expression and increased endothelial permeability, leading to mitochondrial oxidative damage in primary cultured mouse lung vascular endothelial cells (MLVECs). Both the mitochondrial superoxide dismutase 2 mimetic MnTBAP and resveratrol blocked HMGB1-induced mitochondrial oxidative damage, VE-cadherin downregulation, and endothelial hyperpermeability. In in vivo studies, anesthetized male ICR mice were ventilated for 4 h using low tidal volume (6 ml/kg) or high tidal volume (HVT: 30 ml/kg) ventilation. The mice were injected intraperitoneally with resveratrol immediately before the onset of ventilation. We found that resveratrol attenuated HVT-associated lung vascular hyperpermeability and HMGB1 production. HVT caused a significant increase in nuclear factor-erythroid 2-related factor 2 (Nrf2) nuclear translocation and Nrf2 target gene expression in lung tissues, which was further enhanced by resveratrol treatment HMGB1 had no effect on Nrf2 activation, whereas resveratrol treatment activated the Nrf2 signaling pathway in HMGB1-treated MLVECs. Moreover, Nrf2 knockdown reversed the inhibitory effects of resveratrol on HMGB1-induced mitochondrial oxidative damage and endothelial hyperpermeability. The inhibitory effect of resveratrol on cyclic stretch-induced HMGB1 mRNA expression in primary cultured MLVECs was also abolished by Nrf2 knockdown In summary, this study demonstrates that resveratrol protects against lung endothelial barrier dysfunction initiated by HVT. Lung endothelial barrier protection by resveratrol involves inhibition of mechanical stretch-induced HMGB1 release and HMGB1-induced mitochondrial oxidative damage. These protective effects of resveratrol might be mediated through an Nrf2-dependent mechanism. (C) 2015 Elsevier Inc. All rights reserved
引用
收藏
页码:404 / 416
页数:13
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