Application of single molecule detection to DNA sequencing

被引：33

作者：

Goodwin, PM ^{[1
]}

Cai, H ^{[1
]}

Jett, JH ^{[1
]}

IshaugRiley, SL ^{[1
]}

Machara, NP ^{[1
]}

Semin, DJ ^{[1
]}

VanOrden, A ^{[1
]}

Keller, RA ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,LOS ALAMOS,NM 87545

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 5-6期

基金：

美国能源部;

关键词：

D O I：

10.1080/07328319708002914

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A flow cytometric, single molecule approach to DNA sequencing is described. A single, fluorescently labeled DNA fragment is suspended in a flow stream. An exonuclease is added to sequentially cleave the end base into the flow stream where it is detected and identified by laser-induced fluorescence.

引用

页码：543 / 550

页数：8

共 14 条

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