Total phytosterol content in drug materials and extracts from roots of Acanthospermum hispidum by UV-VIS spectrophotometry

被引：57

作者：

Araujo, Larissa B. D. C. ^{[1
]}

Silva, Sarah L. ^{[2
]}

Galvao, Marcos A. M. ^{[3
]}

Ferreira, Magda R. A. ^{[1
]}

Araujo, Evani L. ^{[4
]}

Randau, Karina P. ^{[1
,2
,3
]}

Soares, Luiz A. L. ^{[1
,2
,3
]}

机构：

[1] Univ Fed Pernambuco, Ctr Ciencias Saude, Programa Posgrad Ciencias Farmaceut, Recife, PE, Brazil

[2] Univ Fed Pernambuco, Ctr Ciencias Saude, Dept Ciencias Farmaceut, Lab Farmacognosia, Recife, PE, Brazil

[3] Univ Fed Pernambuco, Ctr Ciencias Biol, Programa Posgrad Inovacao Terapeut, Recife, PE, Brazil

[4] Inst Med Integral, Fac Pernambucana Saude, Recife, PE, Brazil

来源：

REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2013年 / 23卷 / 05期

关键词：

Acanthospermum hispidum; Total phytosterol content; UV-VIS; LIEBERMANN-BURCHARD; QUANTIFICATION; LEAVES; CHOLESTEROL; FLAVONOIDS; NORTHEAST; REGION; PLASMA;

D O I：

10.1590/S0102-695X2013000500004

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Acanthospermum hispidum DC., Asteraceae, is widely used in folk medicine in Brazil to treat respiratory diseases; this biological property has been attributed to its phytosterol content. This study evaluated the spectrophotometric assay method to quantify the total phytosterol content in raw materials and extracts from roots of A. hispidum. The procedure was based on the quantification at 625 nm after the Liebermann-Burchard reaction. The method was evaluated for linearity, repeatability, intermediate precision, accuracy and robustness. The date indicated that the procedure is a valid analytical tool for materials and herbal derivatives from A. hispidum. (C) 2013 Elsevier Editora Ltda. All rights reserved.

引用

页码：736 / 742

页数：7

共 34 条

[1]

Chromatographic analysis of plant sterols in foods and vegetable oils [J].

Abidi, SL .

JOURNAL OF CHROMATOGRAPHY A, 2001, 935 (1-2) :173-201

[2]

Agencia Nacional de Vigilancia Sanitaria-Anvisa, 2003, DIARIO OFICIAL UNIAO

[3]

Agra Maria de Fátima, 2007, Rev. bras. farmacogn., V17, P114, DOI 10.1590/S0102-695X2007000100021

[4]

Survey of medicinal plants used in the region Northeast of Brazil [J].

Agra, Maria de Fatima ;

Silva, Kiriaki Nurit ;

Lima Diniz Basilio, Ionaldo Jose ;

de Freitas, Patricia Franca ;

Barbosa-Filho, Jose Maria .

REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, 2008, 18 (03) :472-508

[5]

[Anonymous], INT J PHARM PHARM SC

[6]

Anvisa, 2010, DIARIO OFICIAL UNIAO

[7]

Araujo ED, 2008, REV BRAS FARMACOGN, V18, P777, DOI 10.1590/S0102-695X2008000500024

[8]

Artiss JD., 2000, HDB LIPOPROTEIN TEST, P189

[9]

Bartos J., 1976, Colorimetric and Fluorimetric Analysis of Steroids

[10]

BEITRAG ZUM CHEMISMUS DER FARBREAKTION NACH LIEBERMANN-BURCHARD [J].

BRIESKORN, CH ;

HOFMANN, H .

ARCHIV DER PHARMAZIE UND BERICHTE DER DEUTSCHEN PHARMAZEUTISCHEN GESSELSCHAFT, 1964, 297 (10) :577-&

← 1 2 3 4 →