Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor

被引:496
作者
Arighi, CN
Hartnell, LM
Aguilar, RC
Haft, CR
Bonifacino, JS
机构
[1] Natl Inst Child Hlth & Human Dev, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA
[2] Natl Inst Diabet & Digest & Kidney Dis, Div Diabet Endocrinol & Metab, NIH, Bethesda, MD 20892 USA
关键词
lysosomal enzymes; multivesicular bodies; sorting nexins; yeast vacuole; clathrin adaptors;
D O I
10.1083/jcb.200312055
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cation-independent mannose 6-phosphate receptor (CI-MPR) mediates sorting of lysosomal hydrolase precursors from the TGN to endosomes. After releasing the hydrolase precursors into the endosomal lumen, the unoccupied receptor returns to the TGN for further rounds of sorting. Here, we show that the mammalian retromer complex participates in this retrieval pathway. The hVps35 subunit of retromer interacts with the cytosolic domain of the CI-MPR. This interaction probably occurs in an endosomal compartment, where most of the retromer is localized. In particular, retromer is associated with tubular-vesicular profiles that emanate from early endosomes or from intermediates in the maturation from early to late endosomes. Depletion of retromer by RNA interference increases the lysosomal turnover of the CI-MPR, decreases cellular levels of lysosomal hydrolases, and causes swelling of lysosomes. These observations indicate that retromer prevents the delivery of the CI-MPR to lysosomes, probably by sequestration into endosome-derived tubules from where the receptor returns to the TGN.
引用
收藏
页码:123 / 133
页数:11
相关论文
共 78 条
[1]   Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells [J].
Barbero, P ;
Bittova, L ;
Pfeffer, SR .
JOURNAL OF CELL BIOLOGY, 2002, 156 (03) :511-518
[2]   A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane [J].
Caplan, S ;
Naslavsky, N ;
Hartnell, LM ;
Lodge, R ;
Polishchuk, RS ;
Donaldson, JG ;
Bonifacino, JS .
EMBO JOURNAL, 2002, 21 (11) :2557-2567
[3]  
CHEN HJ, 1993, J BIOL CHEM, V268, P22338
[4]   Treatment of locked posterior fracture-dislocations of the shoulder by total shoulder arthroplasty [J].
Cheng, SL ;
Mackay, MB ;
Richards, RR .
JOURNAL OF SHOULDER AND ELBOW SURGERY, 1997, 6 (01) :11-17
[5]   Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases [J].
Cooper, AA ;
Stevens, TH .
JOURNAL OF CELL BIOLOGY, 1996, 133 (03) :529-541
[6]   Yeast Gga coat proteins function with clathrin in Golgi to endosome transport [J].
Costaguta, G ;
Stefan, CJ ;
Bensen, ES ;
Emr, SD ;
Payne, GS .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (06) :1885-1896
[7]   BIOSYNTHESIS AND TURNOVER OF THE PHOSPHOMANNOSYL RECEPTOR IN HUMAN-FIBROBLASTS [J].
CREEK, KE ;
SLY, WS .
BIOCHEMICAL JOURNAL, 1983, 214 (02) :353-360
[8]   Syntaxin 6 functions in trans-Golgi network vesicle trafficking [J].
Davanger, S ;
Bock, JB ;
Klumperman, J ;
Scheller, RH .
MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (07) :1261-1271
[9]   GGAs: A family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex [J].
Dell'Angelica, EC ;
Puertollano, R ;
Mullins, C ;
Aguilar, RC ;
Vargas, JD ;
Hartnell, LM ;
Bonifacino, JS .
JOURNAL OF CELL BIOLOGY, 2000, 149 (01) :81-93
[10]   Vps10p transport from the trans-golgi network to the endosome is mediated by clathrin-coated vesicles [J].
Deloche, O ;
Yeung, BG ;
Payne, GS ;
Schekman, R .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (02) :475-485