Zebrafish U6 small nuclear RNA gene promoters contain a SPH element in an unusual location

被引：12

作者：

Halbig, Kari M. ^{[1
]}

Lekven, Arne C. ^{[2
]}

Kunkel, Gary R. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

[2] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

GENE | 2008年 / 421卷 / 1-2期

关键词：

eukaryotic transcription; snRNA gene transcription; transcription element; SBF/Staf/ZNF143;

D O I：

10.1016/j.gene.2008.06.019

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Promoters for vertebrate small nuclear RNA (snRNA) genes contain a relatively simple array of transcriptional control elements, divided into proximal and distal regions. Most of these genes are transcribed by RNA polymerase II (e.g., U1, U2), whereas the U6 gene is transcribed by RNA polymerase III. Previously identified vertebrate U6 snRNA gene promoters consist of a proximal sequence element (PSE) and TATA element in the proximal region, plus a distal region with octamer (OCT) and Sphl postoctamer homology (SPH) elements. We have found that zebrafish U6 snRNA promoters contain the SPH element in a novel proximal position immediately upstream of the TATA element. The zebrafish SPH element is recognized by SPH-binding factor/selenocysteine tRNA gene transcription activating factor/zinc finger protein 143 (SBF/Staf/ZNF143) in vitro. Furthermore, a zebrafish U6 promoter with a defective SPH element is inefficiently transcribed when injected into embryos. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：89 / 94

页数：6

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