Effects of temperature and SDS on the structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus

被引：55

作者：

DAuria, S

Barone, R

Rossi, M

Nucci, R

Barone, G

Fessas, D

Bertoli, E

Tanfani, F

机构：

[1] UNIV NAPLES, DIPARTIMENTO CHIM, I-80131 NAPLES, ITALY

[2] UNIV ANCONA, IST BIOCHIM, FAC MED & CHIRURG, I-60131 ANCONA, ITALY

来源：

BIOCHEMICAL JOURNAL | 1997年 / 323卷

关键词：

D O I：

10.1042/bj3230833

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of temperature and SDS on the three-dimensional organization and secondary structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus were investigated by CD, IR spectroscopy and differential scanning calorimetry. CD spectra in the near UV region showed that the detergent caused a remarkable change in the protein tertiary structure, and far-UV CB analysis revealed only a slight effect on secondary structure. Infrared spectroscopy showed that low concentrations of the detergent (up to 0.02 %) induced slight changes in the enzyme secondary structure, whereas high concentrations caused the alpha-helix content to increase at high temperatures and prevented protein aggregation.

引用

页码：833 / 840

页数：8

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