Granulocyte elastase in cirrhotic patients with spontaneous bacterial peritonitis

Granulocyte elastase (GE) is a powerful proteolytic enzyme that is released by PMNs when degranulated in infectious processes. The aim of this study was to measure GE in ascites and plasma of cirrhotic patients with spontaneous bacterial peritonitis (SBP). We studied 29 cirrhotic patients, 17 of them having SEP (group A). Twelve patients with noninfected ascites formed the control group (group B), At the time of diagnosis of SEP, GE levels in ascites (183.17 +/- 86.11 mu g/liter) and plasma (114.6 I 35.99 mu g/liter) were higher in group A than in group B (27.41 +/- 11.54 mu g/liter, P < 0.00001 and 82.54 +/- 20.52 mu g/liter, P = 0.01, respectively). Levels of GE in ascites had a high value for discriminating between patients with and without SEP. In the patients who responded to the initial antibiotic treatment, these values significantly decreased in ascites (67.69 +/- 54.22 mu g/liter, P = 0.003) and plasma (67 +/- 22.39 mu g/liter, P = 0.01) 48 hr after therapy was started, in parallel with the decrease of PMN in ascites. In patients who did not respond, the production of GE remained elevated. Patients who developed renal insufficiency following SEP had more marked elevation of GE in plasma (144.8 +/- 33.43 mu g/liter) than those with normal renal function (99.5 +/- 27.53 mu g/liter, P = 0.02). These results suggest that the measurement of GE may be helpful for the diagnosis of SEP in patients with cirrhosis and for assessing the efficacy of therapy. In addition, the release of GE into plasma may contribute to the impairment of renal function that follows SEP in some patients.