A unimolecular G-quadruplex that functions as a synthetic transmembrane Na+ transporter

被引：166

作者：

Kaucher, MS ^{[1
]}

Harrell, WA ^{[1
]}

Davis, JT ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 01期

关键词：

D O I：

10.1021/ja056888e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the covalent post-modification of a hydrogen-bonded assembly with the subsequent formation of a potent transmembrane Na+ ion transporter. Olefin metathesis is used to cross-link all 16 guanosine subunits in a lipophilic G-quadruplex. The resulting unimolecular G-quadruplex folds in the environment of a phospholipid membrane and functions as a Na+ ion transporter as judged by fluorescence and 23Na NMR transport assays. Copyright © 2006 American Chemical Society.

引用

页码：38 / 39

页数：2

共 26 条

[1] Covalent capture of dynamic hydrogen-bonded assemblies [J].

Cardullo, F ;

Calama, MC ;

Snellink-Ruël, BHM ;

Weidmann, JL ;

Bielejewska, A ;

Fokkens, R ;

Nibbering, NMM ;

Timmerman, P ;

Reinhoudt, DN .

CHEMICAL COMMUNICATIONS, 2000, (05) :367-368

[2]

Chen L, 1998, TETRAHEDRON LETT, V39, P3627

[3] SUPRAMOLECULAR DESIGN BY COVALENT CAPTURE - DESIGN OF A PEPTIDE CYLINDER VIA HYDROGEN-BOND-PROMOTED INTERMOLECULAR OLEFIN METATHESIS [J].

CLARK, TD ;

GHADIRI, MR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (49) :12364-12365

[4] Diffusion NMR spectroscopy in supramolecular and combinatorial chemistry: An old parameter - New insights [J].

Cohen, Y ;

Avram, L ;

Frish, L .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (04) :520-554

[5] Quadruplex formation by a guanine-rich PNA oligomer [J].

Datta, B ;

Bier, ME ;

Roy, S ;

Armitage, BA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (12) :4199-4207

[6] G-quartets 40 years later:: From 5′-GMP to molecular biology and supramolecular chemistry [J].

Davis, JT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (06) :668-698

[7] Toward artificial ion channels: A lipophilic G-quadruplex [J].

Forman, SL ;

Fettinger, JC ;

Pieraccini, S ;

Gottareli, G ;

Davis, JT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (17) :4060-4067

[8]

Gottarelli G, 1998, ENANTIOMER, V3, P429

[9]

GUPTA RK, 1982, BIOPHYS J, V37, pA76

[10] Binding sites and dynamics of ammonium ions in a telomere repeat DNA quadruplex [J].

Hud, NV ;

Schultze, P ;

Sklenár, V ;

Feigon, J .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (01) :233-243

← 1 2 3 →