Norepinephrine-induced Ca2+ waves depend on InsP3 and ryanodine receptor activation in vascular myocytes

被引:129
作者
Boittin, FX [1 ]
Macrez, N [1 ]
Halet, G [1 ]
Mironneau, J [1 ]
机构
[1] Univ Bordeaux 2, CNRS ESA 5017, Lab Physiol Cellulaire & Pharmacol Mol, F-33076 Bordeaux, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1999年 / 277卷 / 01期
关键词
inositol 1,4,5-trisphosphate receptors; cytosolic calcium; confocal microscopy;
D O I
10.1152/ajpcell.1999.277.1.C139
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In rat portal vein myocytes, Ca2+ signals can be generated by inositol 1,4,5-trisphosphate (InsP(3))- and ryanodine-sensitive Ca2+ release channels, which are located on the same intracellular store. Using a laser scanning confocal microscope associated with the patch-clamp technique, we showed that propagated Ca2+ waves evoked by norepinephrine (in the continuous presence of oxodipine) were completely blocked after internal application of an anti-InsP(3) receptor antibody. These propagated Ca2+ waves were also reduced by similar to 50% and transformed in homogenous Ca2+ responses after application of an antiryanodine receptor antibody or ryanodine. All-or-none Ca2+ waves obtained with increasing concentrations of norepinephrine were transformed in a dose-response relationship with a Hill coefficient close to unity after ryanodine receptor inhibition. Similar effects of the ryanodine receptor inhibition were observed on the norepinephrine- and ACh-induced Ca2+ responses in non-voltage-clamped portal vein and duodenal myocytes and on the norepinephrine-induced contraction. Taken together, these results show that ryanodine-sensitive Ca2+ release channels are responsible for the fast propagation of Ca2+ responses evoked by various neurotransmitters producing InsP(3) in vascular and visceral myocytes.
引用
收藏
页码:C139 / C151
页数:13
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