P53 accumulates in micronuclei after treatment with a DNA breaking chemical, methylnitrosourea, and with the spindle poison, vinblastine

被引：19

作者：

Granetto, C

Ottaggio, L

Abbondandolo, A

Bonatti, S

机构：

[1] NATL INST CANC RES,IST,I-16132 GENOA,ITALY

[2] UNIV GENOA,CHAIR GENET,GENOA,ITALY

[3] CNR,INST MUTAGENESIS & DIFFERENTIAT,IMD,I-56100 PISA,ITALY

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1996年 / 352卷 / 1-2期

关键词：

p53; micronuclei; vinblastine; N-methyl-N-nitrosourea;

D O I：

10.1016/0027-5107(95)00235-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Micronuclei (MN) induced by N-methyl-N-nitrosourea (MNU) or vinblastine in cultured mammalian cells were analyzed for the accumulation of p53 by immunocytochemical staining with a p53 monoclonal antibody. Our data showed that MN induced by both agents were p53-negative at early post-treatment times, but became positive at late times. Assuming that most MNU-induced micronuclei reflect DNA damage, and most vinblastine-induced micronuclei reflect damage to the mitotic apparatus, we conclude that p53 accumulation in micronuclei is not triggered by DNA damage per se but instead probably stems from DNA degradation occurring during ageing of micronuclei.

引用

页码：61 / 64

页数：4

共 8 条

[1]

[Anonymous], 1976, CHEM MUTAGENS PRINCI, DOI DOI 10.1007/978-1-4684-0892-8_1

[2] THE ANALYSIS OF 10 POTENTIAL SPINDLE POISONS FOR THEIR ABILITY TO INDUCE CREST-POSITIVE MICRONUCLEI IN HUMAN-DIPLOID FIBROBLASTS [J].