Nerve growth factor induces angiogenic activity in a mouse model of hindlimb ischemia

被引:69
作者
Turrini, P
Gaetano, C
Antonelli, A
Capogrossi, MC
Aloe, L
机构
[1] CNR, Inst Neurobiol, I-00137 Rome, Italy
[2] Ist Ricovero & Cura Carattere Sci, Ist Dermopat Immacolata, Lab Patol Vasc, I-00167 Rome, Italy
[3] CNR, Inst Neurobiol, I-00137 Rome, Italy
关键词
nerve growth factor; ischemia; hindlimb; arterioles; capillaries; angiogenesis; arteriogenesis;
D O I
10.1016/S0304-3940(02)00090-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Recent studies suggest that nerve growth factor (NGF), a neurotrophic factor known to play a crucial role in neurite growth and differentiation, may also modulate vascular cell functions. In the present study, it was investigated whether NGF exhibits an angiogenic effect in a mouse model of hindlimb ischemia induced by femoral artery occlusion. Enzyme-linked immunosorbent assay determination revealed an enhanced endogenous NGF production (378 +/- 100 and 54 +/- 26 pg/g tissue in 7 day ischemic and normoperfused adductor muscles, respectively; P < 0.05). Furthermore, exogenous NGF, administered subcutaneously for 7 days in ischemic hindlimb, induced a marked increase of arteriole length density (NGF:= 41 +/- 5 vs. Saline = 22 +/- 4 mm/mm(3); p < 0.05). However, capillaries were not significantly increased (NGF = 1035 +/- 182 vs. Saline = 829 +/- 60 mm/mm(3); p > 0.05). In conclusion, the present study provides first evidence that NGF exerts angiogenic properties in vivo. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:109 / 112
页数:4
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