Diazoxide and pinacidil uncouple pyruvate-malate-induced mitochondrial respiration

被引：26

作者：

Kopustinskiene, DM

Jovaisiene, J

Liobikas, J

Toleikis, A

机构：

[1] Kaunas Univ Med, Inst Biomed Res, LT-3007 Kaunas, Lithuania

[2] Vytautas Magnus Univ, Dept Biol, LT-3035 Kaunas, Lithuania

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2002年 / 34卷 / 01期

关键词：

diazoxide; pinacidil; uncoupling; mitochondria; adenine nucleotide translocase;

D O I：

10.1023/A:1013870704002

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We investigated the effects of K-ATP channel openers diazoxide and pinacidil on the respiration rate and membrane potential (Deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 muM) increased the V-2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and decreased the Delta psi by 2-17% and 6-55%. These effects were also similar in the respiration medium without K+. Moreover, carboxyatractyloside completely abolished diazoxide- and pinacidil-induced uncoupling, indicating a role for the mitochondrial adenine nucleotide translocase in this process.

引用

页码：49 / 53

页数：5

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