Comparative analysis of the genome sequences and replication profiles of chikungunya virus isolates within the East, Central and South African (ECSA) lineage

被引：39

作者：

Chen, Karen Caiyun ^{[1
]}

Kam, Yiu-Wing ^{[2
]}

Lin, Raymond Tzer Pin ^{[3
]}

Ng, Mary Mah-Lee ^{[4
]}

Ng, Lisa F. P. ^{[2
]}

Chu, Justin Jang Hann ^{[1
]}

机构：

[1] Natl Univ Singapore, Natl Univ Hlth Syst, Yong Loo Lin Sch Med, Dept Microbiol,Lab Mol RNA Virol & Antiviral Stra, Singapore 117597, Singapore

[2] ASTAR, Singapore Immunol Network, Singapore 138648, Singapore

[3] Coll Med, Minist Hlth, Communicable Dis Div, Natl Publ Hlth Lab, Singapore 169854, Singapore

[4] Natl Univ Singapore, Natl Univ Hlth Syst, Yong Loo Lin Sch Med, Dept Microbiol,Flavivirol Lab, Singapore 117597, Singapore

来源：

VIROLOGY JOURNAL | 2013年 / 10卷

关键词：

Chikungunya virus; Genome sequencing; Virus morphogenesis; Virus replication kinetics; INDIAN-OCEAN; INFECTION; REEMERGENCE; COMPLEXES; OUTBREAK; DOMAIN;

D O I：

10.1186/1743-422X-10-169

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Background: A comparative analysis of the genomic and replication profiles of different geographical chikungunya virus (CHIKV) isolates of the East, Central and South African (ECSA) lineage was performed. Findings: Analysis of the data revealed the different growth kinetics for the different isolates. Deep genome sequencing analysis further revealed specific amino acid mutations in the viral nsP1, nsP3, nsP4, E1 and E2 proteins in the different isolates. Despite the difference in viral genomic profiles, the virus-induced ultrastructural changes within infected cells remained highly conserved among the different chikungunya virus isolates. Conclusions: These findings provide insights into the genomic and replication profiles of the re-emerging chikungunya virus isolates of the ECSA lineage.

引用

页数：8

共 34 条

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