Subgingival microbiota of Brazilian subjects with untreated chronic periodontitis

被引:116
作者
Colombo, APV [1 ]
Teles, RP
Torres, MC
Souto, R
Rosalem, W
Mendes, MCS
Uzeda, M
机构
[1] Univ Fed Rio de Janeiro, Dept Med Microbiol, Rio De Janeiro, Brazil
[2] Univ Estacio de Sa, UNESA, Dept Periodontia, Rio De Janeiro, Brazil
关键词
periodontal diseases/microbiology; periodontitis/microbiology; periodontal diseases/pathogenesis; periodontitis/pathogenesis; DNA probes/methods; comparison studies;
D O I
10.1902/jop.2002.73.4.360
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA-DNA hybridization technique. Methods: Twenty-five periodontitis patients (mean age, 41 +/- 2; mean probing depth [PD], 3.3 +/- 0.2; mean attachment level [AL], 3.6 +/- 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 +/- 0.6; mean PD, 1.8 +/- 0.2; mean AL, 1.7 +/- 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites/tooth. Subgingival plaque samples were obtained from 4 sites in each tooth/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method. Results: Periodontal pathogens, as well as some unusual species (E. faecalis, E. coli and Bartonella sp.), were detected significantly more often and/or in higher levels in the periodontitis group (P<0.05). Most species were more frequently detected in interproximal sites. B. forsythus, P gingivalis, E. nodatum, and F nucleatum ss vincentii showed a significant positive correlation with mean PD and AL (P<0.05). Conclusions: The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species.
引用
收藏
页码:360 / 369
页数:10
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