Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis

被引：16

作者：

Bojak, A ^{[1
]}

Hammer, D ^{[1
]}

Wolf, H ^{[1
]}

Wagner, R ^{[1
]}

机构：

[1] Univ Regensburg, Inst Med Microbiol, D-95053 Regensburg, Germany

来源：

VACCINE | 2002年 / 20卷 / 15期

关键词：

DNA vaccine; tissue specific; HIV-1;

D O I：

10.1016/S0264-410X(02)00081-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Most studies on DNA-based immunization have used viral promoters to drive antigen expression. In this study, we analyzed the properties of the commonly used CMV promoter, the tissue specific murine muscle creatine kinase (MCK) promoter as well as a hybrid MCK/CMV promoter regarding promoter activity and tissue specificity in vitro. Furthermore, the efficiency of inducing HIV-1 Gag specific immune responses in vivo following intramuscular immunization of nacked DNA containing a codon optimized synthetic gene was compared. Although antibody titers and cellular immune responses using the MCK construct were slightly reduced as compared to convential CMV based vector modules, the utilization of nonviral promoters may add significantly to the safety of future DNA vaccines. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1975 / 1979

页数：5

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